148908

Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 1595870..1596525 Replicon chromosome
Accession NZ_CP048243
Organism Ewingella americana strain B6-1

Toxin (Protein)


Gene name higB Uniprot ID A0A2N0NA49
Locus tag GXP68_RS07305 Protein ID WP_034791539.1
Coordinates 1595870..1596211 (+) Length 114 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2N0NA42
Locus tag GXP68_RS07310 Protein ID WP_034791537.1
Coordinates 1596223..1596525 (+) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXP68_RS07280 1590894..1591367 + 474 WP_185689730.1 DUF805 domain-containing protein -
GXP68_RS07285 1591434..1593902 - 2469 WP_185689731.1 type III effector HrpK -
GXP68_RS07290 1594045..1594635 - 591 WP_185689732.1 hypothetical protein -
GXP68_RS07295 1594742..1595440 - 699 WP_185689733.1 RluA family pseudouridine synthase -
GXP68_RS07305 1595870..1596211 + 342 WP_034791539.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GXP68_RS07310 1596223..1596525 + 303 WP_034791537.1 helix-turn-helix transcriptional regulator Antitoxin
GXP68_RS07315 1596620..1597948 - 1329 WP_154146920.1 pyrimidine utilization transport protein G -
GXP68_RS07320 1598034..1598567 - 534 WP_185689734.1 pyrimidine utilization flavin reductase protein F -
GXP68_RS07325 1598580..1599170 - 591 WP_185689735.1 malonic semialdehyde reductase -
GXP68_RS07330 1599155..1599993 - 839 Protein_1434 pyrimidine utilization protein D -
GXP68_RS07335 1600023..1600409 - 387 WP_185689736.1 pyrimidine utilization protein C -
GXP68_RS07340 1600430..1601194 - 765 WP_185689737.1 pyrimidine utilization protein B -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(13-89)


Sequences

Toxin        


Download         Length: 114 a.a.        Molecular weight: 13029.02 Da        Isoelectric Point: 9.1021

>T148908 WP_034791539.1 NZ_CP048243:1595870-1596211 [Ewingella americana]
MWTVLTTDLFDQWFDQQNDGTQEKVLAGLLALRRGGPQLGRPLVDTLCGSGYPHLKELRIQHKGEPLRAFFAFDPFRQAI
VLCAGSKKGKEKQFYKRMLHIADQAYLRHLQEE

Download         Length: 342 bp

>T148908 NZ_CP063900:4507636-4507953 [Klebsiella quasipneumoniae]
ATGTTCAGAATGGTTGTGCATGTGGACGTTAAAAAGGAGTTGCAGGCGCTTCCGGCTATTGTCCAGGCTAAGATGATCAG
GCAATTAGACAAGTTACGCCAAAATCCCACCGCGCTGAGAGAACCTGATAGTAAACCGCTTGGACAAGGGCTATTTGAAA
TTCGAGCGTTAGGTTCGGTACAGGGGCGGGGGATCTACGTTTTTCAGCAAAACAAAACGCTCTTTCTGCTACGGGTTTTT
GTCAAGAAAACGCAAAAAATCCCGTTATCGGAAATTCGCCTGGCGCTGAAGCGATTAGAGGAGATGCGCAATGAGTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 10976.76 Da        Isoelectric Point: 8.5281

>AT148908 WP_034791537.1 NZ_CP048243:1596223-1596525 [Ewingella americana]
MAKNLQDLLAARSSESQKRISEAADEMLLEVKLYALREQLAMSQAAVAKAMGISQPSVVAIEQRGNDIKLSTMKRYVEAM
GGKLRIDVELPDGKHTSFVV

Download         Length: 303 bp

>AT148908 NZ_CP063900:4507946-4508233 [Klebsiella quasipneumoniae]
ATGAGTAAAAAGATCATTGACTGGGATACGTTAAAAACAGAACTGCTTAGCCAGCCTGAGGTTCAGGCTGCGTTCGATGC
GCAAGAACGTAAGCACCGATTACGTACTATGCTGGCACAATGGCGCGAGCAGGCGGGGCTGACCCGAGCCCAGGTGGCGC
AGAGGATGGGGGTGAGTGCGCCAACGGTCTCCAGAATGGAAGCCAATATCACCCGCACCAGCCTGGATACCTTAACGCGA
TACGCGCTAGCGTGCGGTATAAAGCACCCGCAAGTTTTACTTTACTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2N0NA49

Antitoxin

Source ID Structure
AlphaFold DB A0A2N0NA42

References