Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 142505..143232 Replicon chromosome
Accession NZ_CP048243
Organism Ewingella americana strain B6-1

Toxin (Protein)


Gene name higB Uniprot ID A0A377N786
Locus tag GXP68_RS00655 Protein ID WP_034793981.1
Coordinates 142921..143232 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GXP68_RS00650 Protein ID WP_185689023.1
Coordinates 142505..142924 (-) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GXP68_RS00630 138140..138775 - 636 WP_034793993.1 carbonic anhydrase -
GXP68_RS00635 139086..139631 + 546 WP_034793991.1 peptide-methionine (S)-S-oxide reductase MsrA -
GXP68_RS00640 139833..141098 + 1266 WP_185689022.1 bifunctional tetrahydrofolate synthase/dihydrofolate synthase -
GXP68_RS00645 141172..141897 - 726 WP_202034344.1 tetrahydrodipicolinate succinyltransferase -
GXP68_RS00650 142505..142924 - 420 WP_185689023.1 helix-turn-helix domain-containing protein Antitoxin
GXP68_RS00655 142921..143232 - 312 WP_034793981.1 type II toxin-antitoxin system HigB family toxin Toxin
GXP68_RS00660 143432..143881 - 450 WP_185689024.1 GyrI-like domain-containing protein -
GXP68_RS00665 144156..145508 - 1353 WP_185689025.1 glutathione-disulfide reductase -
GXP68_RS00670 145702..146544 - 843 WP_154145719.1 23S rRNA (adenine(2030)-N(6))-methyltransferase RlmJ -
GXP68_RS00675 146730..147320 - 591 WP_185690100.1 histidine phosphatase family protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(20-93)

Antitoxin

Antitoxin HigA

(2-137)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12173.84 Da        Isoelectric Point: 9.3110

>T148903 WP_034793981.1 NZ_CP048243:c143232-142921 [Ewingella americana]
MHVVSKGPFDEAAQKFPNDAKALNDVYRAFKRGNFSDPDEMREVFPSLDRMKYRDKWWVVDVGGNNLRLMFFADFEYGRL
FIKSISTHAEYDKLVKKYRENPA

Download         Length: 312 bp

>T148903 NZ_CP063900:c1292261-1292159 [Klebsiella quasipneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15464.68 Da        Isoelectric Point: 4.4057

>AT148903 WP_185689023.1 NZ_CP048243:c142924-142505 [Ewingella americana]
MISEAIKASQQLVSIVPLLGGSTSKADYKEAVKLAEYLVEFDPSNPLIEMLTAKIDRYENESPDFAEFNALLAATPPGIS
MLRVLMDQYGLTQSDFENEIGQRSLVSRILNGDRNLTVEHMKALAKRFNLPVSIFVNED

Download         Length: 420 bp

>AT148903 NZ_CP063900:1292155-1292302 [Klebsiella quasipneumoniae]
AGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGTTG
GCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGTCTGCG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A377N786

Antitoxin

Source ID Structure

References