Detailed information of TA system TA148898

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 25313..25971
• Replicon: plasmid pD46-3
• Accession: NZ_CP048134
• Organism: Acinetobacter baumannii strain D46

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: KPZ59_RS19470
• Protein ID: WP_000312250.1
• Coordinates: 25612..25971 (-)
• Length: 120 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: KPZ59_RS19465
• Protein ID: WP_001096429.1
• Coordinates: 25313..25612 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
KPZ59_RS19435 (KPZ59_p300034)	20322..20540	+	219	WP_001043201.1	hypothetical protein	-
KPZ59_RS19440 (KPZ59_p300035)	20560..21114	+	555	WP_000790084.1	hypothetical protein	-
KPZ59_RS19445 (KPZ59_p300037)	21416..22384	-	969	WP_012308653.1	IS30 family transposase	-
KPZ59_RS19450 (KPZ59_p300038)	22521..23300	-	780	WP_000422638.1	APH(3')-VI family aminoglycoside O-phosphotransferase	-
KPZ59_RS19455 (KPZ59_p300039)	23401..24369	-	969	WP_012308653.1	IS30 family transposase	-
KPZ59_RS19460 (KPZ59_p300040)	24491..24775	+	285	WP_049592568.1	hypothetical protein	-
KPZ59_RS19465 (KPZ59_p300041)	25313..25612	-	300	WP_001096429.1	XRE family transcriptional regulator	Antitoxin
KPZ59_RS19470 (KPZ59_p300042)	25612..25971	-	360	WP_000312250.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
KPZ59_RS19475 (KPZ59_p300043)	26172..26738	+	567	WP_000710385.1	hypothetical protein	-
KPZ59_RS19480 (KPZ59_p300044)	26787..26969	+	183	WP_000373385.1	hypothetical protein	-
KPZ59_RS19485 (KPZ59_p300045)	27036..27734	+	699	WP_000873188.1	hypothetical protein	-
KPZ59_RS19490 (KPZ59_p300046)	27873..28256	+	384	WP_000654348.1	hypothetical protein	-
KPZ59_RS19495 (KPZ59_p300047)	28323..28901	+	579	WP_228264910.1	hypothetical protein	-
KPZ59_RS19505 (KPZ59_p300050)	29995..30387	+	393	WP_002017443.1	hypothetical protein	-
KPZ59_RS19510 (KPZ59_p300051)	30395..30850	+	456	Protein_45	DEAD/DEAH box helicase family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aph(3')-VIa / blaOXA-23	-	1..74916	74916
	-	inside	IScluster/Tn	aph(3')-VIa / blaOXA-23	-	21416..33631	12215

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (14-109)

Antitoxin

• HigA2-like, helix-turn-helix domain (12-88)

Sequences

Toxin

Protein

• Length: 120 a.a.
• Molecular weight: 13751.62 Da
• Isoelectric Point: 4.8212

>T148898 WP_000312250.1 NZ_CP048134:c25971-25612 [Acinetobacter baumannii]
MAWDVETTELFDSWLAEQDENAQDKILASLLVLSELGPNLGRPHVDTIKESKYPNMKEIRVQVKGHPIRGFFAFDPERKA
IVLCAGDKKGLNEKAFYKEMIKIADEQYEQYLRDNYGDK

Nucleotide

• Length: 360 bp

>T148898 NZ_CP063898:c5426099-5425731 [Klebsiella variicola]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGACGTTGCCGG
TATGCCCGACCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCACCTGCTGGTGATTTCTCGCGGCCATATTTTCAATGATGGAAATAAGCGTACGGCACTGTTT
ATTACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCCGACTTCGTCGGCATGACCGTCGAGGCGGC
AGCGGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGCGGATGA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11012.93 Da
• Isoelectric Point: 10.4856

>AT148898 WP_001096429.1 NZ_CP048134:c25612-25313 [Acinetobacter baumannii]
MAKLNDIISKRSPESQQRIKKMANQMLLDINLSNLREELKISQAEIAKNLGVSQAAISKREKLGKDLKISSLKEYVEAMG
GKVRLDVELPNGKHVAISI

Nucleotide

• Length: 300 bp

>AT148898 NZ_CP063898:c5426317-5426096 [Klebsiella variicola]
ATGAGAACGGTTAACTATAGCGAAGCCCGACAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCGGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATTATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA