Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 4975784..4976386 Replicon chromosome
Accession NZ_CP048107
Organism Escherichia coli strain 201609

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag GWK05_RS23740 Protein ID WP_000897305.1
Coordinates 4976075..4976386 (-) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GWK05_RS23735 Protein ID WP_000356397.1
Coordinates 4975784..4976074 (-) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK05_RS23715 4972286..4973188 + 903 WP_000331377.1 formate dehydrogenase O subunit beta -
GWK05_RS23720 4973185..4973820 + 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
GWK05_RS23725 4973817..4974746 + 930 WP_000027720.1 formate dehydrogenase accessory protein FdhE -
GWK05_RS23730 4974961..4975179 - 219 WP_001298592.1 ribbon-helix-helix domain-containing protein -
GWK05_RS23735 4975784..4976074 - 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
GWK05_RS23740 4976075..4976386 - 312 WP_000897305.1 hypothetical protein Toxin
GWK05_RS23745 4976615..4977523 + 909 WP_001318161.1 alpha/beta hydrolase -
GWK05_RS23750 4977587..4978528 - 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
GWK05_RS23755 4978573..4979010 - 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
GWK05_RS23760 4979007..4979879 - 873 WP_000920762.1 virulence factor BrkB family protein -
GWK05_RS23765 4979873..4980472 - 600 WP_001314324.1 glucose-1-phosphatase -
GWK05_RS23770 4980571..4981356 - 786 WP_000059679.1 DeoR/GlpR family DNA-binding transcription regulator -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T148870 WP_000897305.1 NZ_CP048107:c4976386-4976075 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T148870 NZ_CP063889:4508638-4508740 [Klebsiella variicola]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT148870 WP_000356397.1 NZ_CP048107:c4976074-4975784 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT148870 NZ_CP063889:c4508746-4508602 [Klebsiella variicola]
ACCGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAGT
TGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References