Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yafN-yafO (relBE)/YafO-YafN
Location 4025710..4026404 Replicon chromosome
Accession NZ_CP048107
Organism Escherichia coli strain 201609

Toxin (Protein)


Gene name yafO Uniprot ID Q0T7Q5
Locus tag GWK05_RS19350 Protein ID WP_001263491.1
Coordinates 4025710..4026108 (-) Length 133 a.a.

Antitoxin (Protein)


Gene name yafN Uniprot ID L4JHF0
Locus tag GWK05_RS19355 Protein ID WP_000554755.1
Coordinates 4026111..4026404 (-) Length 98 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWK05_RS19320 4020972..4022429 + 1458 WP_022645227.1 cytosol nonspecific dipeptidase -
GWK05_RS19325 4022438..4022719 + 282 WP_022645226.1 hypothetical protein -
GWK05_RS19330 4022736..4023245 - 510 WP_001361775.1 hydrolase -
GWK05_RS19335 4023307..4023921 - 615 WP_022645225.1 peptide chain release factor H -
GWK05_RS19340 4023918..4025057 - 1140 WP_022645224.1 RNA ligase RtcB family protein -
GWK05_RS19345 4025248..4025700 - 453 WP_023909048.1 GNAT family N-acetyltransferase -
GWK05_RS19350 4025710..4026108 - 399 WP_001263491.1 type II toxin-antitoxin system mRNA interferase toxin YafO Toxin
GWK05_RS19355 4026111..4026404 - 294 WP_000554755.1 type I toxin-antitoxin system antitoxin YafN Antitoxin
GWK05_RS19360 4026456..4027511 - 1056 WP_022645222.1 DNA polymerase IV -
GWK05_RS19365 4027582..4028367 - 786 WP_072224360.1 putative lateral flagellar export/assembly protein LafU -
GWK05_RS19370 4028339..4030051 + 1713 Protein_3790 flagellar biosynthesis protein FlhA -
GWK05_RS19375 4030149..4030922 - 774 WP_022645219.1 C40 family peptidase -
GWK05_RS19380 4031108..4031368 + 261 WP_000729708.1 type II toxin-antitoxin system antitoxin DinJ -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Genomic island - gmhA/lpcA 4025710..4039604 13894


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

mRNA interferase toxin YafO

(27-130)

Antitoxin


No domain identified.



Sequences

Toxin        


Download         Length: 133 a.a.        Molecular weight: 15457.86 Da        Isoelectric Point: 8.0949

>T148865 WP_001263491.1 NZ_CP048107:c4026108-4025710 [Escherichia coli]
MRVFKTKLIRLQLTAEELDALTADFISYKRDGVLPDIFGRDALYDDSFTWPLIKFERVAHIHLANVNNPFPPQLRQFSRT
NDEAHLVYCQGAFDEQAWLLIAILKPEPHKLARDNNQMHKIGKMAEAFRMRF

Download         Length: 399 bp

>T148865 NZ_CP063889:c2485058-2484675 [Klebsiella variicola]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGTACGCGAATGGCGCTAAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTGATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAACTACACCCTGAATAA

Antitoxin


Download         Length: 98 a.a.        Molecular weight: 11235.78 Da        Isoelectric Point: 5.2170

>AT148865 WP_000554755.1 NZ_CP048107:c4026404-4026111 [Escherichia coli]
MHRILAEKSVNITELRKNPAKYFIDQPVAVLSNNRPAGYLLSASAFEALMDMLAEQEEKKLVKARFRPSAARLEEITRRA
EQYLNDMTDDDFNDFKE

Download         Length: 294 bp

>AT148865 NZ_CP063889:c2485300-2485058 [Klebsiella variicola]
ATGATGGCTGAAAGGGATATGGGCAGAATTCTTCTCGATTTATCAGACGATGTCATTCAACGTCTCGATGACCTCAAAGT
GCAGCGTAACATTCCGCGAGCGGAACTGTTACGTGAAGCGGTTGAACAATATCTGGAGAAACAAGATCGAGCTAAAGATA
CCATCTCCAGTGCGCTGGGTCTGTGGCAAGACTGCGAAGAAGACGGCATGGAATATCAACGTCAGCTACGCAAGGAGTGG
TAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A4P7TPK7

Antitoxin

Source ID Structure
AlphaFold DB A0A829G9H5

References