Detailed information of TA system TA148846

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 2174186..2174411
• Replicon: chromosome
• Accession: NZ_CP048107
• Organism: Escherichia coli strain 201609

Toxin (Protein)

• Gene name: hokW
• Uniprot ID: S1ELB5
• Locus tag: GWK05_RS10320
• Protein ID: WP_000813254.1
• Coordinates: 2174256..2174411 (+)
• Length: 52 a.a.

Antitoxin (RNA)

Gene name	sokW
Locus tag	-
Coordinates	2174186..2174244 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK05_RS10275	2169561..2169779	-	219	WP_001171942.1	hypothetical protein	-
GWK05_RS10280	2169939..2170094	-	156	WP_000379575.1	DUF1391 family protein	-
GWK05_RS10285	2170360..2170779	-	420	WP_000362155.1	hypothetical protein	-
GWK05_RS10290	2170880..2171161	+	282	WP_000391950.1	helix-turn-helix domain-containing protein	-
GWK05_RS10295	2171145..2171570	+	426	WP_000693835.1	Rha family transcriptional regulator	-
GWK05_RS10300	2171642..2172712	+	1071	WP_021528583.1	hypothetical protein	-
GWK05_RS10305	2172753..2173178	+	426	WP_001151253.1	DUF977 family protein	-
GWK05_RS10310	2173353..2174018	+	666	WP_000150294.1	epoxyqueuosine reductase QueH	-
-	2174186..2174244	-	59	-	-	Antitoxin
GWK05_RS10320	2174256..2174411	+	156	WP_000813254.1	type I toxin-antitoxin system toxin HokD	Toxin
GWK05_RS10325	2174579..2174857	+	279	WP_024193993.1	hypothetical protein	-
GWK05_RS10330	2174859..2175914	+	1056	WP_065793215.1	DUF968 domain-containing protein	-
GWK05_RS10335	2175915..2176280	+	366	WP_000140004.1	RusA family crossover junction endodeoxyribonuclease	-
GWK05_RS10340	2176277..2176966	+	690	WP_001064894.1	antiterminator	-
GWK05_RS10365	2177763..2177978	+	216	WP_000839572.1	class II holin family protein	-
GWK05_RS10370	2177983..2178333	+	351	WP_065793216.1	YdfR family protein	-
GWK05_RS10375	2178397..2178930	+	534	WP_065793217.1	lysozyme	-
GWK05_RS10380	2179147..2179329	+	183	WP_077575695.1	Rz1 family lipoprotein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2161150..2203241	42091

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (6-47)

Sequences

Toxin

Protein

• Length: 52 a.a.
• Molecular weight: 5736.89 Da
• Isoelectric Point: 6.1531

>T148846 WP_000813254.1 NZ_CP048107:2174256-2174411 [Escherichia coli]
MKQQKAMLIALIVICLTVIVTALVTRKDLCEVRIRTGQTEVAVFTAYEPEE

Nucleotide

• Length: 156 bp

>T148846 NZ_CP063885:1959271-1959654 [Klebsiella pneumoniae]
ATGACCTCCGGATCTGCGCTTTTTGATACCAATATTCTTATTGATTTATTTAGCGGGCGTCGCGAAGCCAAACAGGCGCT
GGAGGCCTGGCCACCGCAGAATGCGATCAGTCTGATTACCTGGATGGAGGTGATGGTTGGCGCTAAAAAATATCACCAGG
AGCAGCGCACGCGAATGGCGCTGAGCACCTTTAATATCATTAACATCTCACAGGATATTGCAGAGCGAAGCGTTGCGCTG
CGGCAGGAGTATAAGCTTAAGCTGCCGGATGCCATCATTCTGGCGACGGCGCAACTCCATCGTCTGGAACTAATTACGCG
GAATACGAAGGATTTTGCCGGTATTCCTGGCGTAGTCACGCCGTACGAAATCCACCCTGAATAA

Antitoxin

Nucleotide

• Length: 59 bp

>AT148846 NZ_CP048107:c2174244-2174186 [Escherichia coli]
CCTTGCCTTTCGGCACGTAAGAGGCTAACCTACATGTGTTCAGCATGGATTGAGCCTCA

Structures

Toxin

• AlphaFold DB: A0A4P7TMW0

Antitoxin