Detailed information of TA system TA148841

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): prlF-yhaV (relBE)/YhaV-PrlF
• Location: 684705..685504
• Replicon: chromosome
• Accession: NZ_CP048107
• Organism: Escherichia coli strain 201609

Toxin (Protein)

• Gene name: yhaV
• Uniprot ID: V0SSH7
• Locus tag: GWK05_RS03255
• Protein ID: WP_000347273.1
• Coordinates: 684705..685169 (-)
• Length: 155 a.a.

Antitoxin (Protein)

• Gene name: prlF
• Uniprot ID: D6JF08
• Locus tag: GWK05_RS03260
• Protein ID: WP_001308975.1
• Coordinates: 685169..685504 (-)
• Length: 112 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK05_RS03225	679706..680140	-	435	WP_000948824.1	PTS sugar transporter subunit IIA	-
GWK05_RS03230	680158..681036	-	879	WP_001298314.1	PTS system mannose/fructose/sorbose family transporter subunit IID	-
GWK05_RS03235	681026..681805	-	780	WP_000406214.1	PTS mannose/fructose/sorbose/N-acetylgalactosamine transporter subunit IIC	-
GWK05_RS03240	681816..682289	-	474	WP_001295547.1	PTS N-acetylgalactosamine transporter subunit IIB	-
GWK05_RS03245	682312..683592	-	1281	WP_023908831.1	tagatose-bisphosphate aldolase subunit KbaZ	-
GWK05_RS03250	683841..684650	+	810	WP_000072187.1	aga operon transcriptional regulator AgaR	-
GWK05_RS03255	684705..685169	-	465	WP_000347273.1	type II toxin-antitoxin system ribonuclease toxin YhaV	Toxin
GWK05_RS03260	685169..685504	-	336	WP_001308975.1	type II toxin-antitoxin system antitoxin PrlF	Antitoxin
GWK05_RS03265	685653..687224	-	1572	WP_023908830.1	galactarate dehydratase	-
GWK05_RS03270	687599..688934	+	1336	Protein_642	galactarate/glucarate/glycerate transporter GarP	-
GWK05_RS03275	688950..689720	+	771	WP_001058227.1	2-dehydro-3-deoxyglucarate aldolase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin endonuclease, YhaV (12-150)

Antitoxin

• PrlF antitoxin (14-110)

Sequences

Toxin

Protein

• Length: 155 a.a.
• Molecular weight: 17836.25 Da
• Isoelectric Point: 9.6924

>T148841 WP_000347273.1 NZ_CP048107:c685169-684705 [Escherichia coli]
MDFPQRVNGWALYAHPCFQETYDALVAEVETLKGKDPENYQRKAATKLLAVVHKVIEEHITVNPSSPAFRHGKSLGSGKN
KDWSRVKFGAGRYRLFFRYSEKEKVIILGWMNDENTLRTYGKKTDAYTVFSKMLKRGHPPADWETLTRETEETH

Nucleotide

• Length: 465 bp

>T148841 NZ_CP063885:c81013-80840 [Klebsiella pneumoniae]
GTGAAACAGCGCGAATTCCAGCGCTGGTTGGAATCTCAGGGGGTCGAAATCACTGACGGTGCAAAACATCTGAAGTTGAG
ACTGAACGGGAAGCGTAGCGTCATGCCTCGCCACCCGGGATCCGAGATTGTAGAACAGCTTCGTAAAGCTATTCTCAAAC
AACTAGGCCTGTAG

Antitoxin

Protein

• Length: 112 a.a.
• Molecular weight: 12343.97 Da
• Isoelectric Point: 4.8616

>AT148841 WP_001308975.1 NZ_CP048107:c685504-685169 [Escherichia coli]
MPANARSHAVLTTESKVTIRGQTTIPAPVREALKLKPGLDSIHYEILPGGQVFMCRLGDEQEDHTMNAFLRFLDADIQNN
PQKTRPFNIQQGKKLVAGMDVNIDDEIGDDE

Nucleotide

• Length: 336 bp

>AT148841 NZ_CP063885:c80781-80371 [Klebsiella pneumoniae]
ATGCGATATCCTGTAAACCTAAGTAAAGCTGAAGAAGGCGGATTCTTCGTAGAATTCCCGGATATACCTGAAGCTCTTAC
GCAGGGTGATACCAAAGAAGAAGCGCTTGATAACGCACTTGACGCTTTGGTAACGGCTTTCGAGTTTTACTTCGAAGATG
GAGATAGGATTCCGGAGCCTGGTGTACCGACAGGAGACTATGTAGAGTTACCGTTAAGTATTGAAGCTAAAGTATTAATG
TTAAATGCCTTTATTGAATCAAAATTGAGCCAAACTGAACTGGCTGCAAAAATGAATGTTAAAAAACAGGAAGTTACAAG
GTTGTTTGACCTTAAACATTCAACAAAAATTGATACAATTCAAAAGGCTGTCAACTCACTTGGACATAGAATGGAGTTAA
AGTATGCGTAA

Structures

Toxin

• AlphaFold DB: V0SSH7

Antitoxin

• AlphaFold DB: D6JF08