Detailed information of TA system TA148831

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/COG4683-HTH_37
• Location: 3540041..3540654
• Replicon: chromosome
• Accession: NZ_CP048071
• Organism: Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)

• Gene name: higB
• Uniprot ID: O53332
• Locus tag: tig002_RS16700
• Protein ID: WP_003899954.1
• Coordinates: 3540041..3540385 (+)
• Length: 115 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: tig002_RS16705
• Protein ID: WP_031705302.1
• Coordinates: 3540382..3540654 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
tig002_RS16665	3535114..3535974	+	861	WP_003416628.1	alpha/beta hydrolase	-
tig002_RS16670	3535949..3536464	+	516	WP_003899950.1	nitroreductase family deazaflavin-dependent oxidoreductase	-
tig002_RS16680	3536704..3536997	+	294	WP_003416635.1	hypothetical protein	-
tig002_RS16685	3537285..3538574	+	1290	WP_003416640.1	ATP-binding protein	-
tig002_RS16690	3538921..3539355	-	435	WP_003899952.1	type II toxin-antitoxin system VapC family toxin	-
tig002_RS16695	3539358..3539810	-	453	WP_003899953.1	type II toxin-antitoxin system Phd/YefM family antitoxin	-
tig002_RS16700	3540041..3540385	+	345	WP_003899954.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
tig002_RS16705	3540382..3540654	+	273	WP_031705302.1	helix-turn-helix transcriptional regulator	Antitoxin
tig002_RS16715	3542168..3542515	+	348	WP_003899956.1	DUF2384 domain-containing protein	-
tig002_RS16720	3542512..3543132	+	621	Protein_3284	RES family NAD+ phosphorylase	-
tig002_RS16725	3543292..3544557	-	1266	WP_031705187.1	hypothetical protein	-
tig002_RS16730	3544725..3544934	+	210	WP_003416778.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-like (12-103)

Antitoxin

• Cro/C1-type helix-turn-helix domain (38-90)

Sequences

Toxin

Protein

• Length: 115 a.a.
• Molecular weight: 12692.50 Da
• Isoelectric Point: 5.6920

>T148831 WP_003899954.1 NZ_CP048071:3540041-3540385 [Mycobacterium tuberculosis]
MAVILLPQVERWFFALNRDAMASVTGAIDLLEMEGPTLGRPVVDKVNDSTFHNMKELRPAGTSIRILFAFDPARQAILLL
GGDKAGNWKRWYDNNIPIADQRSENWLASEHGGG

Nucleotide

• Length: 345 bp

>T148831 NZ_CP063881:2781958-2782326 [Klebsiella pneumoniae]
ATGACGCTGCAGATTATCTCAGCGGAAGAGATAATACAGTTTCACGACAGGCTGCTCCGCGTCACGCCTGGCGTTGCCGG
TATGCCCGATCCGGGGCGTGCCGAAGCGATAATGTATAGGGTGCTAAACAAAATTGAATATGAAGGTGTGACAGACGTGT
GGCGACTCGCTGCGATGCATCTGCTGGCGATTTCTCGCGGTCATATATTTAATGATGGTAATAAGCGTACGGCACTGTTT
ATCACCCTGCTTTTTTTAAAGCGAAATGGAATTATATTGCCAGCGAATCCAGACTTCGTCGGCATGACCGTCGAGGCAGC
AGCAGGGCAACTTACCCTGGAACAGATTGTCGCGCGTTTGCGTGGATGA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 9807.23 Da
• Isoelectric Point: 10.1922

>AT148831 WP_031705302.1 NZ_CP048071:3540382-3540654 [Mycobacterium tuberculosis]
MTMARNWRDIRADAVAQGRVDLQRAAVAREEMRDAVLAHRLAEIRKALGHARQADVAALMGVSQARVSKLESGDLSHTEL
GTLQAYVAAL

Nucleotide

• Length: 273 bp

>AT148831 NZ_CP063881:2781740-2781961 [Klebsiella pneumoniae]
ATGAGAACGGTTAACTATAGCGAAGCCCGGCAAAATCTGGCCGATGTGCTGGAAAGCGCAGTGACAGGTGTACCTGTGAC
CATTACCCGTCGTGGGCATAAATCTGCAGTCATCATTAGTGCAGAAGAGTTTGAACGCTACCAGGCGGCCAGAATGGATG
ATGAGTTCGCGGCTATCATGGCGGTTCATGGTGATGAGATCAGGGAGCTTGCGGATAAATGA

Structures

Toxin

• AlphaFold DB: A0A7U4FBK2