Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2262942..2263861 Replicon chromosome
Accession NZ_CP048071
Organism Mycobacterium tuberculosis strain RW-TB008

Toxin (Protein)


Gene name HigB2 Uniprot ID L7N4R2
Locus tag tig002_RS10600 Protein ID WP_003900449.1
Coordinates 2263256..2263861 (-) Length 202 a.a.

Antitoxin (Protein)


Gene name HigA2 Uniprot ID L7N5K9
Locus tag tig002_RS10595 Protein ID WP_003410124.1
Coordinates 2262942..2263247 (-) Length 102 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
tig002_RS10565 2257953..2259209 - 1257 WP_031704872.1 HNH endonuclease -
tig002_RS10570 2259563..2260138 + 576 WP_003410100.1 hypothetical protein -
tig002_RS10575 2260135..2261175 + 1041 WP_003410103.1 ImmA/IrrE family metallo-endopeptidase -
tig002_RS10580 2261417..2262136 + 720 WP_003410108.1 DUF433 domain-containing protein -
tig002_RS10585 2262126..2262542 + 417 WP_003410114.1 hypothetical protein -
tig002_RS10590 2262558..2262857 - 300 WP_003410120.1 hypothetical protein -
tig002_RS10595 2262942..2263247 - 306 WP_003410124.1 XRE family transcriptional regulator Antitoxin
tig002_RS10600 2263256..2263861 - 606 WP_003900449.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
tig002_RS10605 2263886..2264245 - 360 WP_003410131.1 hypothetical protein -
tig002_RS10610 2264405..2265082 - 678 WP_169035333.1 hypothetical protein -
tig002_RS10615 2265034..2265270 - 237 WP_003410133.1 hypothetical protein -
tig002_RS10620 2265510..2266373 - 864 WP_169035334.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(86-181)

Antitoxin

HigA2-like, helix-turn-helix domain

(32-92)


Sequences

Toxin        


Download         Length: 202 a.a.        Molecular weight: 22744.96 Da        Isoelectric Point: 7.3073

>T148814 WP_003900449.1 NZ_CP048071:c2263861-2263256 [Mycobacterium tuberculosis]
VNVPWENAHGGALYCLIRGDEFSAWHRLLFQRPGCAESVLACRHFLDGSPVARCSYPEEYHPCVISRIALLCDSVGWTAD
VERISAWLNGLDRETYELVFAAIEVLEEEGPALGCPLVDTVRGSRHKNMKELRPGSQGRSEVRILFAFDPARQAIMLAAG
NKAGRWTQWYDEKIKAADEMFAEHLAQFEDTKPKRRKRKKG

Download         Length: 606 bp

>T148814 NZ_CP063878:c1033579-1032662 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCCATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCGCAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGCGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAATCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCGATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin


Download         Length: 102 a.a.        Molecular weight: 11291.91 Da        Isoelectric Point: 10.7482

>AT148814 WP_003410124.1 NZ_CP048071:c2263247-2262942 [Mycobacterium tuberculosis]
MAMTLRDMDAVRPVNREAVDRHKARMRDEVRAFRLRELRAAQSLTQVQVAALAHIRQSRVSSIENGDIGSAQVNTLRKYV
SALGGELDITVRLGDETFTLA

Download         Length: 306 bp

>AT148814 NZ_CP063878:c1034343-1033585 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGAGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A7U4BV20

Antitoxin

Source ID Structure
PDB 7EWC
PDB 7EWD
PDB 7EWE
AlphaFold DB A0A7U4BV30

References