Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-RelB
Location 139713..140249 Replicon plasmid Ps8942B-p4
Accession NZ_CP048060
Organism Piscirickettsia salmonis strain Ps-8942B

Toxin (Protein)


Gene name yoeB Uniprot ID A0A1L6THY5
Locus tag GW537_RS17755 Protein ID WP_036772441.1
Coordinates 139713..139985 (-) Length 91 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID A0A0B8U9U2
Locus tag GW537_RS17760 Protein ID WP_032126795.1
Coordinates 139989..140249 (-) Length 87 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GW537_RS17730 134904..135633 + 730 Protein_181 IS6 family transposase -
GW537_RS17735 135676..136503 - 828 WP_048876208.1 IS30 family transposase -
GW537_RS17740 136916..137095 + 180 WP_157894752.1 hypothetical protein -
GW537_RS17745 137275..138339 + 1065 WP_174872554.1 IS91 family transposase -
GW537_RS17750 138909..139637 - 729 WP_017375910.1 IS6 family transposase -
GW537_RS17755 139713..139985 - 273 WP_036772441.1 Txe/YoeB family addiction module toxin Toxin
GW537_RS17760 139989..140249 - 261 WP_032126795.1 type II toxin-antitoxin system Phd/YefM family antitoxin Antitoxin
GW537_RS17765 140589..140753 + 165 Protein_188 IS6 family transposase -
GW537_RS17770 141039..141605 + 567 Protein_189 IS6 family transposase -
GW537_RS17775 141671..142303 - 633 Protein_190 IS6-like element ISPsa2 family transposase -
GW537_RS17780 142373..142540 + 168 Protein_191 IS6 family transposase -
GW537_RS17785 142591..142797 - 207 Protein_192 IS6 family transposase -
GW537_RS17790 142881..143696 - 816 WP_036775032.1 hypothetical protein -
GW537_RS17795 143744..144433 - 690 WP_017375909.1 hypothetical protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)
- inside Non-Mobilizable plasmid - - 1..208102 208102
- inside IScluster/Tn - - 129291..147566 18275


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(7-88)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(5-74)


Sequences

Toxin        


Download         Length: 91 a.a.        Molecular weight: 10785.31 Da        Isoelectric Point: 7.8926

>T148769 WP_036772441.1 NZ_CP048060:c139985-139713 [Piscirickettsia salmonis]
MSKLNLLTWSDDAWDDYLYWQDTDKKVLKKINKLIKECQRMPTEGIGHPEPLKHDFSGYWSRKIKGEHRLVYSFDATSLM
ISACRYHYDQ

Download         Length: 273 bp

>T148769 NZ_CP063867:c3359865-3359581 [Klebsiella variicola]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGATGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCCGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 87 a.a.        Molecular weight: 9555.93 Da        Isoelectric Point: 5.1167

>AT148769 WP_032126795.1 NZ_CP048060:c140249-139989 [Piscirickettsia salmonis]
MLRDNINATAARDNFKSTCDTVCENNAPVLVKRKGAEDVVIISASDFGGMEETIHLLSSRIMATRLMRSMKQLDEGKGVQ
RDIIEE

Download         Length: 261 bp

>AT148769 NZ_CP063867:c3360097-3359855 [Klebsiella variicola]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGACATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCGGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A1L6THY5

Antitoxin

Source ID Structure
AlphaFold DB A0A0B8U9U2

References