Detailed information of TA system TA148735

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 116805..117231
• Replicon: plasmid pTEM1-GZEC065
• Accession: NZ_CP048026
• Organism: Escherichia coli strain GZEC065

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GWG09_RS23565
• Protein ID: WP_001312861.1
• Coordinates: 116805..116963 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	117007..117231 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG09_RS23520	111976..112521	-	546	WP_000059851.1	type-F conjugative transfer system pilin assembly thiol-disulfide isomerase TrbB	-
GWG09_RS23525	112508..112792	-	285	WP_000624109.1	type-F conjugative transfer system pilin chaperone TraQ	-
GWG09_RS23530	112873..113187	+	315	WP_001324103.1	hypothetical protein	-
GWG09_RS23535	113189..113536	-	348	WP_001287910.1	conjugal transfer protein TrbA	-
GWG09_RS23540	113552..114295	-	744	WP_001030393.1	type-F conjugative transfer system pilin assembly protein TraF	-
GWG09_RS24685	114288..114431	-	144	Protein_117	conjugal transfer protein TrbE	-
GWG09_RS23545	114478..115182	-	705	WP_001067858.1	IS6-like element IS26 family transposase	-
GWG09_RS23550	115246..115479	-	234	Protein_119	DUF932 domain-containing protein	-
GWG09_RS23555	115597..115884	-	288	WP_000107535.1	hypothetical protein	-
GWG09_RS23565	116805..116963	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	117007..117231	-	225	NuclAT_0	-	Antitoxin
-	117007..117231	-	225	NuclAT_0	-	Antitoxin
-	117007..117231	-	225	NuclAT_0	-	Antitoxin
-	117007..117231	-	225	NuclAT_0	-	Antitoxin
GWG09_RS23570	117043..117231	+	189	WP_001299721.1	hypothetical protein	-
GWG09_RS23575	117243..117962	-	720	WP_001276238.1	plasmid SOS inhibition protein A	-
GWG09_RS23580	117959..118393	-	435	WP_000845936.1	conjugation system SOS inhibitor PsiB	-
GWG09_RS23585	118448..118645	-	198	Protein_125	hypothetical protein	-
GWG09_RS23590	118673..118906	-	234	WP_000005987.1	DUF905 family protein	-
GWG09_RS23595	118974..119513	-	540	WP_000290840.1	single-stranded DNA-binding protein	-
GWG09_RS24690	119539..119745	-	207	WP_000275856.1	hypothetical protein	-
GWG09_RS23610	120884..121855	-	972	WP_000817028.1	ParB/RepB/Spo0J family plasmid partition protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / mph(A) / aph(3')-Ia / aadA2 / dfrA12 / oqxB / oqxA / tet(A) / blaTEM-1B / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..142236	142236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T148735 WP_001312861.1 NZ_CP048026:c116963-116805 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T148735 NZ_CP063861:23948-24106 [Klebsiella pneumoniae]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT148735 NZ_CP048026:c117231-117007 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin