Detailed information of TA system TA148730

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 91364..91618
• Replicon: plasmid pTEM1-GZEC065
• Accession: NZ_CP048026
• Organism: Escherichia coli strain GZEC065

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: -
• Locus tag: GWG09_RS23435
• Protein ID: WP_001351576.1
• Coordinates: 91364..91570 (-)
• Length: 69 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	91557..91618 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWG09_RS23400	86913..87314	-	402	WP_001398199.1	type II toxin-antitoxin system toxin endoribonuclease PemK	-
GWG09_RS23405	87247..87504	-	258	WP_000557619.1	type II toxin-antitoxin system antitoxin PemI	-
GWG09_RS23410	87597..88250	-	654	WP_064055666.1	CPBP family intramembrane metalloprotease	-
GWG09_RS23415	89192..90049	-	858	WP_000774297.1	incFII family plasmid replication initiator RepA	-
GWG09_RS23420	90042..90524	-	483	WP_001273588.1	hypothetical protein	-
GWG09_RS23425	90517..90591	-	75	WP_001442103.1	RepA leader peptide Tap	-
GWG09_RS23430	90823..91080	-	258	WP_000083845.1	replication regulatory protein RepA	-
GWG09_RS23435	91364..91570	-	207	WP_001351576.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	91557..91618	+	62	NuclAT_1	-	Antitoxin
-	91557..91618	+	62	NuclAT_1	-	Antitoxin
-	91557..91618	+	62	NuclAT_1	-	Antitoxin
-	91557..91618	+	62	NuclAT_1	-	Antitoxin
GWG09_RS23440	91980..93521	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
GWG09_RS23445	93536..94282	+	747	WP_001016257.1	IS21-like element ISEc12 family helper ATPase IstB	-
GWG09_RS24680	94462..94536	-	75	Protein_97	endonuclease	-
GWG09_RS23450	94782..94994	-	213	WP_001299730.1	ANR family transcriptional regulator	-
GWG09_RS23455	95130..95690	-	561	WP_000139315.1	fertility inhibition protein FinO	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / mph(A) / aph(3')-Ia / aadA2 / dfrA12 / oqxB / oqxA / tet(A) / blaTEM-1B / sitABCD	iroN / iroE / iroD / iroC / iroB / iutA / iucD / iucC / iucB / iucA	1..142236	142236

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (23-64)

Sequences

Toxin

Protein

• Length: 69 a.a.
• Molecular weight: 7826.31 Da
• Isoelectric Point: 8.8807

>T148730 WP_001351576.1 NZ_CP048026:c91570-91364 [Escherichia coli]
MKYLNTTDCSLFFAERSKFMTKYALIGLLAVCATVLCFSLIFRERLCELNIHRGNTVVQVTLAYEARK

Nucleotide

• Length: 207 bp

>T148730 NZ_CP063858:3493361-3493894 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTGTTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Nucleotide

• Length: 62 bp

>AT148730 NZ_CP048026:91557-91618 [Escherichia coli]
TTAAGGTACTTCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Antitoxin