Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HTH(antitoxin)
Location 3261401..3262003 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name higB Uniprot ID U9XIS6
Locus tag GWG09_RS15695 Protein ID WP_000897305.1
Coordinates 3261401..3261712 (+) Length 104 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GWG09_RS15700 Protein ID WP_000356397.1
Coordinates 3261713..3262003 (+) Length 97 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS15665 3256431..3257216 + 786 WP_000059678.1 DeoR/GlpR family DNA-binding transcription regulator -
GWG09_RS15670 3257315..3257914 + 600 WP_001295269.1 glucose-1-phosphatase -
GWG09_RS15675 3257908..3258780 + 873 WP_000920762.1 virulence factor BrkB family protein -
GWG09_RS15680 3258777..3259214 + 438 WP_000560983.1 D-tyrosyl-tRNA(Tyr) deacylase -
GWG09_RS15685 3259259..3260200 + 942 WP_001297068.1 fatty acid biosynthesis protein FabY -
GWG09_RS15690 3260264..3261172 - 909 WP_001162704.1 alpha/beta hydrolase -
GWG09_RS15695 3261401..3261712 + 312 WP_000897305.1 hypothetical protein Toxin
GWG09_RS15700 3261713..3262003 + 291 WP_000356397.1 helix-turn-helix domain-containing protein Antitoxin
GWG09_RS15705 3262589..3262807 + 219 WP_001611167.1 ribbon-helix-helix domain-containing protein -
GWG09_RS15710 3263026..3263268 + 243 WP_001086388.1 hypothetical protein -
GWG09_RS15715 3263598..3264527 - 930 WP_000027708.1 formate dehydrogenase accessory protein FdhE -
GWG09_RS15720 3264524..3265159 - 636 WP_000829013.1 formate dehydrogenase cytochrome b556 subunit -
GWG09_RS15725 3265156..3266058 - 903 WP_000331377.1 formate dehydrogenase O subunit beta -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin


No domain identified.


Antitoxin

Cro/C1-type helix-turn-helix domain

(36-85)


Sequences

Toxin        


Download         Length: 104 a.a.        Molecular weight: 12190.19 Da        Isoelectric Point: 9.7791

>T148724 WP_000897305.1 NZ_CP048025:3261401-3261712 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Download         Length: 312 bp

>T148724 NZ_CP063858:c912516-912414 [Klebsiella pneumoniae]
GCAAGGCGACTTAGCCTGCATTAATGCCAACTTTTAGCGCACGGCTCTCTCCCAAGAGCCATTTCCCTGGACCGAATACA
GGAATCGTATTCGGTCTCTTTTT

Antitoxin


Download         Length: 97 a.a.        Molecular weight: 10846.43 Da        Isoelectric Point: 10.8907

>AT148724 WP_000356397.1 NZ_CP048025:3261713-3262003 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Download         Length: 291 bp

>AT148724 NZ_CP063858:912407-912552 [Klebsiella pneumoniae]
TAAAGATAAAAAGAGACCGAATACGATTCCTGTATTCGGTCCAGGGAAATGGCTCTTGGGAGAGAGCCGTGCGCTAAAAG
TTGGCATTAATGCAGGCTAAGTCGCCTTGCACTATAAGAATAGTTTAACGCGTCAGCTTTTCCAGT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0E0Y7I7

Antitoxin

Source ID Structure

References