Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) yefM-yoeB (relBE)/YoeB-YefM
Location 1226851..1227353 Replicon chromosome
Accession NZ_CP048025
Organism Escherichia coli strain GZEC065

Toxin (Protein)


Gene name yoeB Uniprot ID U9YDH0
Locus tag GWG09_RS06075 Protein ID WP_000767829.1
Coordinates 1226851..1227105 (-) Length 85 a.a.

Antitoxin (Protein)


Gene name yefM Uniprot ID S1Q063
Locus tag GWG09_RS06080 Protein ID WP_001259255.1
Coordinates 1227102..1227353 (-) Length 84 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWG09_RS06045 1221866..1222093 - 228 WP_000985273.1 sulfurtransferase TusA family protein -
GWG09_RS06050 1222107..1223165 - 1059 WP_000492340.1 transport protein YeeE -
GWG09_RS06055 1223344..1224702 - 1359 WP_000019197.1 putrescine/proton symporter PlaP -
GWG09_RS06060 1224692..1224754 - 63 WP_010723108.1 membrane protein YoeI -
GWG09_RS06065 1224969..1225898 - 930 WP_063078859.1 LysR family transcriptional regulator -
GWG09_RS06070 1225944..1226768 - 825 WP_000754737.1 SDR family oxidoreductase -
GWG09_RS06075 1226851..1227105 - 255 WP_000767829.1 type II toxin-antitoxin system mRNA interferase toxin YoeB Toxin
GWG09_RS06080 1227102..1227353 - 252 WP_001259255.1 YoeB-YefM toxin-antitoxin system antitoxin YefM Antitoxin
GWG09_RS06085 1227636..1227686 + 51 WP_001364200.1 his operon leader peptide -
GWG09_RS06090 1227832..1228731 + 900 WP_000131782.1 ATP phosphoribosyltransferase -
GWG09_RS06095 1228737..1230041 + 1305 WP_001572810.1 histidinol dehydrogenase -
GWG09_RS06100 1230038..1231108 + 1071 WP_063078858.1 histidinol-phosphate transaminase -
GWG09_RS06105 1231108..1232175 + 1068 WP_063078857.1 bifunctional histidinol-phosphatase/imidazoleglycerol-phosphate dehydratase HisB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin YoeB

(2-84)

Antitoxin

Type II toxin-antitoxin system, antitoxin Phd/YefM

(1-73)


Sequences

Toxin        


Download         Length: 85 a.a.        Molecular weight: 10183.58 Da        Isoelectric Point: 8.0353

>T148716 WP_000767829.1 NZ_CP048025:c1227105-1226851 [Escherichia coli]
VKLIWSEESWDDYLYWQETDKRIVKKINELIKDTRRTPFEGKGKPEPLKHNLSGFWSRRITEEHRLVYAVTDDSLLIAAC
RYHY

Download         Length: 255 bp

>T148716 NZ_CP063856:c3232996-3231701 [Klebsiella pneumoniae]
GTGGAAAGATTAACGGTTGCGCTAAATGGCATCATTGTTGGTACGCTGGAGAAAACAGCGGGCGGTGAAATGTCTTTTTA
CTACCAAAGAGACTGGCTGGAAAGAGCTGGTTCTCGTGCTATTTCTCTTTCGATGCCGTTACGGGAAGAGCGTTATCGTG
GCGAAATCGTTTATAACTTTTTTGATAATCTTTTGCCAGATAGTGAGCAAATTCGTAGCCGGATTCAGGCGCGGTTCCAT
GCGCCCACAAAGCAACCTTTCGACTTGTTAGCACGTATTGGGATGGACTGTGTTGGGGCTATACAGCTTTGGCCTTATGA
AACCGATATTCCATCTATTCAGAGAGTGGATGCCGAACCCTTAACTGAAAACGAAATAGAACATCTGCTGCAGGGATACA
AAGATGCTCCATTGGGTATGCAGGCAGAGGTCGATGATTTTCGTATTTCACTGGCGGGTGCACAGGAAAAAACAGCTCTG
CTCTGGTGGAATAATCGATGGTGTAGGCCACGCGGGAGCACACCGACAAGCCATATTTTTAAACTACCTATTGGCTATAT
CGCCAATAACGGAATTGACCTTCGCGAGAGCGTTGAAAATGAGTGGCTGTGTTCAAAAATAGCGGCTGAGTTTGGTTTTC
CCGTTGCGGATACCCGAGTTGCGTATTTTGGGCAACAAAAGATACTGATTGTTGAACGTTTCGATCGGCGATGGGCTAAA
GATGGTCAGTGGCTGATGCGTTTGCCAACGGAAGACTTTTGTCAGGCATTGGGTGTAGCTCCGGCGCTTAAGTATGAATC
TGATGGTGGGCCAGGAATCGCCAGAAGCATGGATTTGTTACTGGGTTCTCAACAACCGCTTAAGGACAGGATTTTGTTTT
TCAGAAGTCAGATCTTTTTCTGGATGTTGGCGGCAATTGATGGCCATGCCAAAAACTTCAGTGTATTCCTTGAACCAAAC
AGCGCTTACCGTATGGCCCCGCTTTACGATGTAATGTCTGCTTACCCTTTGATGCACCCGAAAGGTATTCCTCCCAGAAA
AGCTAAAATGGCGATGGCGCTATCCGGACGTAATCGCCATTTTCACTGGAGTACAATCATGCCCCGTCATTTTCTTTCCA
CTGCGCGAGAAGTGAGCTTTCCCGTTTTGCAGGTTGAGCAATTGCTGGCGGAGATGAAACAACGAACTGAATCGGTGATC
TCAAAAGTGGAAACGATGTTACCTGCAGGATTCCCAGAGCACATAAGCGAAGCTATTTTTGAAGGATTAAGGCATCAGTC
AGCGCGTATTCCGTAG

Antitoxin


Download         Length: 84 a.a.        Molecular weight: 9307.58 Da        Isoelectric Point: 4.7954

>AT148716 WP_001259255.1 NZ_CP048025:c1227353-1227102 [Escherichia coli]
MRTISYSEARQNLSATMMKAVEDHAPILITRQNGEACVLMSLEEYNSLEETAYLLRSPANARRLMDSIDSLKSGKGTEKD
IIE

Download         Length: 252 bp

>AT148716 NZ_CP063856:c3233251-3233000 [Klebsiella pneumoniae]
ATGAACGTAACCTCACCCAAAATGCTGGCTCAGGCAGTCAGGGATGCACGCAAGCAACGTAAGCTGACTCAACGCGAAGC
TGCTGAAAGTATGAGCATGAAGCAATCAACGGTTTCAGAGTTTGAGAACCATCCGGAGGGGACACGGCTGGACACGCTTT
TTAAGCTGCTTGCCGCTCTGGATCTTGAACTTCAGGTCGTAAGCCGAAAGGGTAAAAACAAAACATCTGAGGGTTGGGAC
CAGGAGTGGTGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
PDB 6NY6
PDB 2A6S
PDB 6OXA
PDB 2A6R
PDB 4V8X
PDB 6OTR
PDB 6OXI
PDB 2A6Q

Antitoxin

Source ID Structure
PDB 2A6Q
AlphaFold DB A0A7U9LNR0

References