Detailed information of TA system TA148681

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 34782..35021
• Replicon: plasmid p3575-CTX-M
• Accession: NZ_CP048011
• Organism: Escherichia coli strain RUT3575

Toxin (Protein)

• Gene name: srnB
• Uniprot ID: A0A762TWR7
• Locus tag: GW576_RS23310
• Protein ID: WP_023144756.1
• Coordinates: 34782..34916 (-)
• Length: 45 a.a.

Antitoxin (RNA)

Gene name	srnC
Locus tag	-
Coordinates	34961..35021 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GW576_RS23280	30483..31340	-	858	WP_000130640.1	incFII family plasmid replication initiator RepA	-
GW576_RS23285	31333..31407	-	75	WP_031943482.1	RepA leader peptide Tap	-
GW576_RS23290	31404..31538	-	135	Protein_40	protein CopA/IncA	-
GW576_RS23295	31769..33310	+	1542	WP_001298859.1	IS21-like element ISEc12 family transposase	-
GW576_RS23300	33325..34071	+	747	WP_053906593.1	IS21-like element ISEc12 family helper ATPase IstB	-
GW576_RS23305	34231..34485	-	255	WP_000083850.1	replication regulatory protein RepA	-
GW576_RS23310	34782..34916	-	135	WP_023144756.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	34961..35021	+	61	NuclAT_0	-	Antitoxin
-	34961..35021	+	61	NuclAT_0	-	Antitoxin
-	34961..35021	+	61	NuclAT_0	-	Antitoxin
-	34961..35021	+	61	NuclAT_0	-	Antitoxin
GW576_RS23315	34988..35274	-	287	Protein_45	DUF2726 domain-containing protein	-
GW576_RS23320	35787..35999	-	213	WP_013023861.1	hypothetical protein	-
GW576_RS23325	36130..36690	-	561	WP_000139341.1	fertility inhibition protein FinO	-
GW576_RS23330	36745..37491	-	747	WP_000205718.1	conjugal transfer pilus acetylase TraX	-
GW576_RS23335	37511..39904	-	2394	Protein_49	AAA family ATPase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	tet(B) / sul1 / qacE / aadA5 / blaTEM-1B / blaCTX-M-15 / blaOXA-1 / aac(6')-Ib-cr	-	1..76937	76937

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (4-44)

Sequences

Toxin

Protein

• Length: 45 a.a.
• Molecular weight: 4896.90 Da
• Isoelectric Point: 7.7209

>T148681 WP_023144756.1 NZ_CP048011:c34916-34782 [Escherichia coli]
MTKYTLIGLLAVCATVLCFSLIFREQLCELNIHRGNTVVQVTLA

Nucleotide

• Length: 135 bp

>T148681 NZ_CP063843:3913606-3913983 [Morganella morganii]
ATGAATACATTACCCGACACTCACGTACGGGAGGCATCGGGCTGCCCGTCTCCCGTCACCATCTGGCAGACACTGCTCAC
CCGACTGCTGGACCAGCACTATGGCCTCACGCTGAATGACACACCGTTCGCTGATGAACGTGTGATTGAGCAGCATATTG
AGGCAGGGATTTCACTGTGTGACGCGGTGAACTTTCTCGTTGAAAAATACGCGCTGGTACGTACCGACCAGCCGGGATTC
AGCGCAGGAGCCCCATCGCAGTTAATCAACAGCATTGATATTCTCCGGGCTCGCAGGGCGACCGGCCTGATGACCCGGAA
CAATTACAGAATGGTAAATAACATTACCCAGGGCAAGCATCCGGAGGCAAAACGATGA

Antitoxin

Nucleotide

• Length: 61 bp

>AT148681 NZ_CP048011:34961-35021 [Escherichia coli]
TAAAGTGCATCATGCAGACCTCACGAGTTAATGGATTAACGAGTGGGGTCTTCGCATTTCT

Structures

Toxin

• AlphaFold DB: A0A762TWR7

Antitoxin