Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) DinJ-YafQ (relBE)/YafQ-DinJ
Location 2681681..2682245 Replicon chromosome
Accession NZ_CP048003
Organism Lacticaseibacillus paracasei strain CACC 566

Toxin (Protein)


Gene name yafQ Uniprot ID A0A510WIJ9
Locus tag GWC93_RS12800 Protein ID WP_016363448.1
Coordinates 2681681..2681983 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name dinJ Uniprot ID K6SLT0
Locus tag GWC93_RS12805 Protein ID WP_003570051.1
Coordinates 2681976..2682245 (-) Length 90 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GWC93_RS12785 2676994..2677551 + 558 WP_003584278.1 helix-turn-helix domain-containing protein -
GWC93_RS12790 2677976..2680153 - 2178 WP_003570057.1 RNA-binding transcriptional accessory protein -
GWC93_RS12795 2680389..2681081 - 693 WP_003570055.1 hypothetical protein -
GWC93_RS15905 2681199..2681342 - 144 WP_162259784.1 hypothetical protein -
GWC93_RS12800 2681681..2681983 - 303 WP_016363448.1 type II toxin-antitoxin system YafQ family toxin Toxin
GWC93_RS12805 2681976..2682245 - 270 WP_003570051.1 type II toxin-antitoxin system RelB/DinJ family antitoxin Antitoxin
GWC93_RS12810 2682619..2683272 - 654 WP_003570049.1 GNAT family N-acetyltransferase -
GWC93_RS12815 2683317..2683946 - 630 WP_003570047.1 DUF2399 domain-containing protein -
GWC93_RS12820 2684009..2684335 - 327 WP_003584272.1 YrdB family protein -
GWC93_RS12825 2684328..2684732 - 405 WP_003570045.1 MerR family transcriptional regulator -
GWC93_RS12830 2684930..2686156 + 1227 WP_003570043.1 hypothetical protein -
GWC93_RS12835 2686353..2687240 + 888 WP_003570041.1 ABC transporter ATP-binding protein -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, YafQ-like toxin

(3-97)

Antitoxin

RelB antitoxin/Antitoxin DinJ

(10-70)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11510.33 Da        Isoelectric Point: 9.8881

>T148648 WP_016363448.1 NZ_CP048003:c2681983-2681681 [Lacticaseibacillus paracasei]
MYSLVPTPTFKRDLKRLSKKHWPMDELKTAVNLLAAGTNAELLSKKYADHALSSSSEWKGYRELYVDGPRGDWLLIYKIE
QQDLILTLVRTGSHHNLLVK

Download         Length: 303 bp

>T148648 NZ_CP063829:c1609043-1608864 [Streptococcus pneumoniae]
ATGCCTATGACACAAAAAGAGATGGTTAAACTCCTTACGGCCCATGGTTGGATAAAGACTAGAGGCGGTAAAGGCTCCCA
TATTAAAATGGAGAAGCAAGGGGAAAGACTTATCACAATCCCTCATGGTGAGCTGAATAAGTACACTGAAAGAGGGATAA
GAAAGCAAGCTGGGTTGTAA

Antitoxin


Download         Length: 90 a.a.        Molecular weight: 9982.68 Da        Isoelectric Point: 7.2026

>AT148648 WP_003570051.1 NZ_CP048003:c2682245-2681976 [Lacticaseibacillus paracasei]
MAATKKETRLNIRVDPELKSAAQIVANDMGIDLTAAVTMFMTKMVKDHALPFTPTSLPVETLQALKEAKHPELLKKYSTP
DDMWRDLNV

Download         Length: 270 bp

>AT148648 NZ_CP063829:c1608827-1608375 [Streptococcus pneumoniae]
ATGTTAGTTACGTATCCAGCTTTATTTTATTATGACGACACAGACGGAACAGAAGCGACTTATTTTGTCCATTTCCCAGA
TTTTGAATACTCAGCTACACAAGGAGAGGGGATTTCTGAGGCTTTGGCTATGGGGTCGGAGTGGCTAGGGATAACTGTTG
CAGATTTGATAGAGAGTGATGGAGAGTTACCTCAGCCATCAGATATCAATAGCTTGTCTTTAATTGATAATGATCCTTTT
AAAGATGATGAAGATTTCGTGTCAACCTATGACCTTGATAAATCTTTTATTTCTATGGTATCTGTTGATGTATCAGAATA
CCTAGGAAGTCAGGAACCCATTAAAAAGACTTTGACTATACCAAAATGGGCAGATAAGTTGGGACGAGAAATGGGACTTA
ACTTTTCTCAGACTTTGACAGACGCTATTGCAGATAAGAAAGTTCAAGCCTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A510WIJ9

Antitoxin

Source ID Structure
AlphaFold DB A0A0E2BRK6

References