Detailed information of TA system TA148625

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 4460..5045
• Replicon: plasmid pDETAB3
• Accession: NZ_CP047976
• Organism: Acinetobacter baumannii strain DETAB-P2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N8ZPD7
• Locus tag: GWK28_RS19660
• Protein ID: WP_000897309.1
• Coordinates: 4725..5045 (-)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GWK28_RS19655
• Protein ID: WP_000369780.1
• Coordinates: 4460..4732 (-)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK28_RS19630	144..1094	+	951	WP_001208778.1	replication initiation protein RepM	-
GWK28_RS19635	1087..1662	+	576	WP_017725651.1	helix-turn-helix domain-containing protein	-
GWK28_RS19640	1683..1826	+	144	WP_004786602.1	hypothetical protein	-
GWK28_RS19645	2111..3319	-	1209	WP_000343018.1	IS256-like element ISAba26 family transposase	-
GWK28_RS19650	3398..3727	-	330	WP_000610966.1	four-helix bundle copper-binding protein	-
GWK28_RS19655	4460..4732	-	273	WP_000369780.1	helix-turn-helix domain-containing protein	Antitoxin
GWK28_RS19660	4725..5045	-	321	WP_000897309.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GWK28_RS19665	5232..5429	-	198	WP_000476222.1	hypothetical protein	-
GWK28_RS19670	5496..5723	-	228	WP_000921865.1	hypothetical protein	-
GWK28_RS19675	5822..6037	-	216	WP_000071892.1	cold-shock protein	-
GWK28_RS19680	6363..6794	-	432	WP_000390052.1	hypothetical protein	-
GWK28_RS19685	6812..7078	-	267	WP_006581723.1	hypothetical protein	-
GWK28_RS19690	7289..8725	+	1437	WP_119027411.1	MobA/MobL family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	-	-	1..9132	9132
	-	flank	IS/Tn	-	-	2111..3319	1208

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12209.01 Da
• Isoelectric Point: 7.9835

>T148625 WP_000897309.1 NZ_CP047976:c5045-4725 [Acinetobacter baumannii]
MLFIETSIFTKQIKELVSDEEYRQLQQDLLVQPDKGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETSILRQLVKEQFHG

Nucleotide

• Length: 321 bp

>T148625 NZ_CP063804:c2812049-2811630 [Mycobacterium orygis]
GTGACGGCACTGCTCGATGTCAATGTGCTGATCGCGCTGGGCTGGCCGAATCACGTTCACCATGCGGCCGCGCAGCGATG
GTTCACGCAGTTCTCCTCGAATGGGTGGGCCACCACGCCGATCACCGAGGCAGGGTATGTCCGAATTTCAAGCAATCGCA
GTGTGATGCAGGTGTCGACCACGCCGGCTATCGCGATCGCTCAGTTGGCGGCGATGACTTCTCTTGCCGGGCACACGTTT
TGGCCTGACGATGTGCCACTGATCGTTGGGAGCGCCGGCGATCGCGATGCGGTGTCCAACCACCGTCGGGTCACCGACTG
CCATCTCATCGCCTTGGCCGCGCGCTACGGGGGCCGGTTGGTCACATTCGATGCCGCACTGGCCGATTCAGCATCCGCAG
GCCTCGTCGAGGTGTTGTAG

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10122.63 Da
• Isoelectric Point: 8.6582

>AT148625 WP_000369780.1 NZ_CP047976:c4732-4460 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEIKASRVTELELPDIKEVREKTGLTQAEFAARLHISARTLQNWEQGRRYPTGPAATLI
RILDAHPTLI

Nucleotide

• Length: 273 bp

>AT148625 NZ_CP063804:c2812270-2812046 [Mycobacterium orygis]
ATGCGCACCACGTTGCAGATTGATGATGATGTTCTAGAAGATGCTCGTAGCATCGCGCGGTCGGAGGGCAAGTCAGTCGG
CGCGGTAATTTCTGAGTTGGCGCGTAGGTCGCTCCGTCCGGTTGGGATTGTCGAGGTTGACGGATTTCCGGTTTTTGATG
TTCCGCCGGATGCGCCGACGGTGACTTCCGAGGATGTCGTCCGCGCGCTCGAGGACGACGTGTGA

Structures

Toxin

• AlphaFold DB: A0A855WJQ3