Detailed information of TA system TA148624

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/RelE-HTH
• Location: 81117..81702
• Replicon: plasmid pDETAB2
• Accession: NZ_CP047975
• Organism: Acinetobacter baumannii strain DETAB-P2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: N9CJF7
• Locus tag: GWK28_RS19505
• Protein ID: WP_000897307.1
• Coordinates: 81117..81437 (+)
• Length: 107 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GWK28_RS19510
• Protein ID: WP_000369781.1
• Coordinates: 81430..81702 (+)
• Length: 91 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GWK28_RS19490	77415..78929	+	1515	WP_032073148.1	MFS transporter	-
GWK28_RS19495	79033..80139	-	1107	WP_032073131.1	two-component sensor histidine kinase AdeS	-
GWK28_RS19500	80129..80857	-	729	WP_032073130.1	efflux system response regulator transcription factor AdeR	-
GWK28_RS19505	81117..81437	+	321	WP_000897307.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GWK28_RS19510	81430..81702	+	273	WP_000369781.1	helix-turn-helix domain-containing protein	Antitoxin
GWK28_RS19515	82195..83670	+	1476	WP_000052512.1	ABC-F type ribosomal protection protein Msr(E)	-
GWK28_RS19520	83726..84610	+	885	WP_000155092.1	Mph(E) family macrolide 2'-phosphotransferase	-
GWK28_RS19525	84803..85414	-	612	WP_015060246.1	recombinase family protein	-
GWK28_RS19530	85609..85989	+	381	WP_015060210.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Non-Mobilizable plasmid	aac(3)-IId / blaOXA-58 / blaNDM-1 / sul2 / msr(E) / mph(E) / tet(39)	-	1..100072	100072
	-	flank	IS/Tn	msr(E) / mph(E) / tet(39)	-	82195..89692	7497

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-2 (41-102)

Antitoxin

• Cro/C1-type helix-turn-helix domain (37-85)

Sequences

Toxin

Protein

• Length: 107 a.a.
• Molecular weight: 12187.95 Da
• Isoelectric Point: 7.1639

>T148624 WP_000897307.1 NZ_CP047975:81117-81437 [Acinetobacter baumannii]
MLFIETSIFTKQIKDLVSDEEYRQLQQDLLVQPDRGDLIKNGGGIRKVRCAQGNKGKSGGIRVIYYWVTEDDQIFFLVAY
PKSVKDNLTDKETAILHQLVKEQFHG

Nucleotide

• Length: 321 bp

>T148624 NZ_CP063804:2765659-2766084 [Mycobacterium orygis]
GTGGCGCTGCTCGACGTCAACGCATTGGTCGCGCTGGCGTGGGACTCACACATCCACCACGCCCGGATCCGCGAGTGGTT
TACCGCCAACGCCACGCTCGGCTGGGCGACTTGCCCGCTCACCGAAGCCGGCTTCGTGCGGGTGTCGACGAACCCAAAAG
TACTTCCCAGCGCGATCGGGATCGCAGACGCTCGACGGGTCCTCGTGGCACTACGCGCCGTGGGAGGCCACCGCTTCCTG
GCTGACGACGTATCGCTCGTCGATGACGATGTTCCGTTGATCGTCGGTTATCGCCAGGTGACCGACGCCCATCTGCTGAC
ACTCGCCCGCCGGCGCGGCGTCCGCCTGGTCACCTTCGACGCCGGTGTCTTCACCCTCGCCCAACAACGCCCCAAGACGC
CAGTGGAGCTGCTGACCATCCTCTAA

Antitoxin

Protein

• Length: 91 a.a.
• Molecular weight: 10149.61 Da
• Isoelectric Point: 8.6582

>AT148624 WP_000369781.1 NZ_CP047975:81430-81702 [Acinetobacter baumannii]
MDNNLFDDLVASIKEAGAIKRKEVKASRVTELELPDIKEVREKTGLSQNEFAARLHISPRTLQNWEQGRRYPTGPAATLI
RILDAHPSLI

Nucleotide

• Length: 273 bp

>AT148624 NZ_CP063804:2765432-2765653 [Mycobacterium orygis]
GTGCGAACGACACTTGACCTTGACGACGATGTGATCGCCGCGGCACGTGAACTTGCCTCCAGCCAGCGCCGCTCGCTCGG
CTCGGTGATTTCCGAACTCGCACGCCGTGGTCTCATGCCCGGACGCGTCGAGGCTGACGACGGGCTGCCGGTGATCCGCG
TTCCAGCCGGGACCCCGCCGATCACACCGGAGATGGTCCGTCGCGCGCTCGATGAGGACTGA

Structures

Toxin

• AlphaFold DB: A0A0A8TMZ3