Detailed information of TA system TA148607

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3877721..3878396
• Replicon: chromosome
• Accession: NZ_CP047965
• Organism: Enterobacter hormaechei strain 189

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A7T9SGM4
• Locus tag: GUY47_RS18660
• Protein ID: WP_032654718.1
• Coordinates: 3877721..3878020 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUY47_RS18665
• Protein ID: WP_032654721.1
• Coordinates: 3878031..3878396 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY47_RS18650	3874098..3876290	+	2193	WP_032654716.1	type I secretion system permease/ATPase	-
GUY47_RS18655	3876310..3877470	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GUY47_RS18660	3877721..3878020	+	300	WP_032654718.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GUY47_RS18665	3878031..3878396	+	366	WP_032654721.1	HigA family addiction module antidote protein	Antitoxin
GUY47_RS18670	3878423..3879604	-	1182	WP_017382758.1	PLP-dependent aminotransferase family protein	-
GUY47_RS18675	3879625..3880512	-	888	WP_032654725.1	LysR family transcriptional regulator	-
GUY47_RS18680	3880610..3881212	+	603	WP_032654728.1	short chain dehydrogenase	-
GUY47_RS18685	3881209..3881940	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-95)

Antitoxin

• Cro/C1-type helix-turn-helix domain (20-71)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11582.25 Da
• Isoelectric Point: 9.8425

>T148607 WP_032654718.1 NZ_CP047965:3877721-3878020 [Enterobacter hormaechei]
MINHYRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SAGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T148607 NZ_CP063804:c1396195-1395926 [Mycobacterium orygis]
GTGAGCGACGACCATCCCTACCACGTGGCGATCACCGCGACAGCGGCACGCGACCTGCAACGCTTACCCGAAAAGATCGC
CGCCGCATGTGTCGAGTTTGTTTTCGGACCGCTGCTTAACAACCCGCATAGGTTGGGCAAGCCGCTGCGCAATGACCTTG
AAGGCCTCCACTCAGCCCGCCGCGGTGATTACCGCGTCGTCTACGCCATCGACGACGGCCACCACCGAGTCGAGATCATC
CACATCGCTCGTCGCAGTGCCAGCTACTGA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13731.81 Da
• Isoelectric Point: 9.5936

>AT148607 WP_032654721.1 NZ_CP047965:3878031-3878396 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKLGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT148607 NZ_CP063804:c1396461-1396192 [Mycobacterium orygis]
ATGGCTGTTGTCCCACTGGGCGAAGTCCGCAATCGCCTCTCTGAGTACGTCGCCGAAGTTGAGCTGACACACGAGCGCAT
CACGATAACCCGGCACGGTCATCCGGCGGCGGTATTGATCTCGGCCGATGACCTGGCGTCCATCGAGGAAACGCTGGAGG
TGCTACGCACCCCTGGCGCCAGCGAGGCCATTCGTGAAGGCCTCGCCGATGTTGCCGCAGGGCGCTTCGTGAGCAACGAC
GAGATCCGCAACCGTTACACCGCGCGGTGA

Structures

Toxin

• AlphaFold DB: A0A7T9SGM4