Detailed information of TA system TA148596

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1407118..1407845
• Replicon: chromosome
• Accession: NZ_CP047965
• Organism: Enterobacter hormaechei strain 189

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A8B3V067
• Locus tag: GUY47_RS06880
• Protein ID: WP_022650547.1
• Coordinates: 1407534..1407845 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUY47_RS06875
• Protein ID: WP_032652900.1
• Coordinates: 1407118..1407537 (-)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUY47_RS06860	1404189..1405310	-	1122	WP_032652893.1	glutamate--cysteine ligase	-
GUY47_RS06865	1405416..1406306	+	891	WP_032652895.1	oxidoreductase	-
GUY47_RS06870	1406360..1406794	-	435	WP_032652897.1	hypothetical protein	-
GUY47_RS06875	1407118..1407537	-	420	WP_032652900.1	helix-turn-helix domain-containing protein	Antitoxin
GUY47_RS06880	1407534..1407845	-	312	WP_022650547.1	type II toxin-antitoxin system HigB family toxin	Toxin
GUY47_RS06885	1408538..1408957	+	420	WP_032655562.1	hypothetical protein	-
GUY47_RS06890	1409071..1409526	-	456	WP_032652902.1	hypothetical protein	-
GUY47_RS06895	1409817..1410698	-	882	WP_003858850.1	LysR family transcriptional regulator	-
GUY47_RS06900	1410804..1411583	+	780	WP_026094386.1	acetolactate decarboxylase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (20-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (83-136)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12554.34 Da
• Isoelectric Point: 9.9435

>T148596 WP_022650547.1 NZ_CP047965:c1407845-1407534 [Enterobacter hormaechei]
MHIVSRAPFDTAARHYPNQAQALDDLYRVLKKEHYPSPDEMRKRFPSLDRMKYREKWWVIDVGGGHLRVMFFADFERGKF
FIKHISTHAEYDKLTDYYRRTKE

Nucleotide

• Length: 312 bp

>T148596 NZ_CP063804:718820-719215 [Mycobacterium orygis]
ATGGTGATCGACACGTCCGCGCTCGTTGCGATGCTCAGCGACGAGCCAGACGCAGAGCGGTTCGAGGCCGCCGTCGAAGC
CGACCACATCCGGCTGATGTCGACGGCGTCTTACCTGGAAACGGCACTCGTGATAGAAGCCCGCTTCGGTGAACCGGGCG
GACGTGAGCTGGATCTGTGGCTTCATCGCGCCGCGGTCGACCTTGTTGCCGTGCATGCCGACCAAGCGGATGCCGCGCGC
GCCGCCTACCGCACGTACGGCAAGGGAAGGCATCGTGCGGGGCTCAACTACGGCGACTGCTTCTCATACGGCCTCGCCAA
GATCAGCGGCCAGCCACTCCTGTTCAAGGGCGAAGATTTCCAACACACCGACATCGCCACGGTCGCGCTGCCCTAA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15437.48 Da
• Isoelectric Point: 4.6051

>AT148596 WP_032652900.1 NZ_CP047965:c1407537-1407118 [Enterobacter hormaechei]
MNYAAAIKAANALTNELPFLGSSPSRQDYEDALALVEYLIEHDPDNPLVEMLTAKIDKYENESSEFAEFNARIASIPSGV
ALLRTLMDQYQLTQSDFENEIGKKSLVSRILNGQRTLTLDHMRALAKRFGLPVSAFVGN

Nucleotide

• Length: 420 bp

>AT148596 NZ_CP063804:718566-718820 [Mycobacterium orygis]
ATGGCGCTGAGTATCAAGCACCCGGAAGCCGACCGGCTCGCGCGAGCGCTTGCGGCGCGCACCGGCGAGACGTTGACCGA
GGCAGTGGTTACCGCGTTGCGCGAGCGGCTCGCTCGTGAGACTGGGCGTGCCCGTGTTGTCCCGTTGCGCGACGAGCTTG
CCGCGATTCGGCACCGGTGCGCAGCGTTGCCGGTGGTCGACAACCGGTCCGCTGAGGCGATTCTCGGCTATGACGAGCGC
GGATTGCCGGCCTGA

Structures

Toxin

• AlphaFold DB: A0A8B3V067