Detailed information of TA system TA148568

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 1184257..1184843
• Replicon: chromosome
• Accession: NZ_CP047943
• Organism: Synechococcus sp. RS9909

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: SynRS9909_RS05760
• Protein ID: WP_186593814.1
• Coordinates: 1184565..1184843 (-)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: SynRS9909_RS05755
• Protein ID: WP_186593813.1
• Coordinates: 1184257..1184556 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
SynRS9909_RS05710	1179492..1179710	-	219	WP_186593804.1	hypothetical protein	-
SynRS9909_RS05715	1180078..1180428	-	351	WP_186593805.1	hypothetical protein	-
SynRS9909_RS05720	1180604..1180840	+	237	WP_186593806.1	DNA-binding protein	-
SynRS9909_RS05725	1180827..1181249	+	423	WP_186593807.1	type II toxin-antitoxin system VapC family toxin	-
SynRS9909_RS05730	1181293..1181559	-	267	WP_186593808.1	hypothetical protein	-
SynRS9909_RS05735	1181579..1182178	-	600	WP_186593809.1	hypothetical protein	-
SynRS9909_RS05740	1183044..1183361	+	318	WP_186593810.1	nucleotidyltransferase domain-containing protein	-
SynRS9909_RS05745	1183358..1183732	+	375	WP_186593811.1	DUF86 domain-containing protein	-
SynRS9909_RS05750	1183823..1184041	-	219	WP_186593812.1	DUF433 domain-containing protein	-
SynRS9909_RS05755	1184257..1184556	-	300	WP_186593813.1	HigA family addiction module antidote protein	Antitoxin
SynRS9909_RS05760	1184565..1184843	-	279	WP_186593814.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
SynRS9909_RS05765	1184936..1185874	+	939	WP_186593837.1	hypothetical protein	-
SynRS9909_RS05770	1185931..1186365	+	435	WP_186593815.1	hypothetical protein	-
SynRS9909_RS05775	1186376..1186906	+	531	WP_186593816.1	hypothetical protein	-
SynRS9909_RS05780	1186955..1187197	+	243	WP_186593817.1	hypothetical protein	-
SynRS9909_RS05785	1187240..1187914	+	675	WP_186593818.1	hypothetical protein	-
SynRS9909_RS05790	1187921..1188445	+	525	WP_186593819.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (1-92)

Antitoxin

• Cro/C1-type helix-turn-helix domain (16-68)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10703.24 Da
• Isoelectric Point: 10.3672

>T148568 WP_186593814.1 NZ_CP047943:c1184843-1184565 [Synechococcus sp. RS9909]
MIRSFRCPDTERLAQGWAVPRFQSFERVARRKLRQLEIAGRLDDLRVPPGNRLEALRGDRAGQHSIRINDQFRLCFIWTA
AGPDAVEIVDYH

Nucleotide

• Length: 279 bp

>T148568 NZ_CP063775:c44164-43859 [Escherichia coli O25b:H4-ST131]
ATGCAGTTTAAGGTTTACACCTATAAAAGAGAGAGCCGTTATCGTCTGTTTGTGGATGTACAGAGTGATATTATTGACAC
GCCCGGGCGACGGATGGTGATCCCCCTGGCCAGTGCACGTCTGCTGTCAGATAAAGTCTCCCGTGAACTTTACCCGGTGG
TGCATATCGGGGATGAAAGCTGGCGCATGATGACCACCGATATGGCCAGTGTGCCGGTCTCCGTTATCGGGGAAGAAGTG
GCTGATCTCAGCCACCGCGAAAATGACATCAAAAACGCCATTAACCTGATGTTCTGGGGAATATAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 10807.38 Da
• Isoelectric Point: 4.5338

>AT148568 WP_186593813.1 NZ_CP047943:c1184556-1184257 [Synechococcus sp. RS9909]
MNRLPPIPPGELLQEEFLQPLGVSQYRLAKAIGVPASRISEIVTGQRAITADTDLRLCRFLGLSPGYWLRAQAAHDTELA
SAELADELEQIQPLVAAQT

Nucleotide

• Length: 300 bp

>AT148568 NZ_CP063775:c44384-44166 [Escherichia coli O25b:H4-ST131]
ATGAAGCAGCGTATTACAGTGACAGTTGACAGCGACAGCTATCAGTTGCTCAAGGCATATGATGTCAATATCTCCGGTCT
GGTAAGCACAACCATGCAGAATGAAGCCCGTCGTCTGCGTGCCGAACGCTGGAAAGCGGAAAATCAGGAAGGGATGGCTG
AGGTCGCCCGGTTTATTGAAATGAACGGCTCTTTTGCTGACGAGAACAGGGACTGGTGA