Detailed information of TA system TA148494

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 4172619..4173205
• Replicon: chromosome
• Accession: NZ_CP047897
• Organism: Nibribacter ruber strain BT10

Toxin (Protein)

• Gene name: graT
• Uniprot ID: -
• Locus tag: GU926_RS17625
• Protein ID: WP_160694233.1
• Coordinates: 4172924..4173205 (-)
• Length: 94 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GU926_RS17620
• Protein ID: WP_160694231.1
• Coordinates: 4172619..4172918 (-)
• Length: 100 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GU926_RS17595	4168592..4169377	+	786	WP_160694221.1	heme ABC transporter ATP-binding protein	-
GU926_RS17600	4169525..4170667	-	1143	WP_160694223.1	MFS transporter	-
GU926_RS17605	4170975..4171409	-	435	WP_160694225.1	hypothetical protein	-
GU926_RS17610	4171574..4171945	-	372	WP_160694227.1	fluoride efflux transporter CrcB	-
GU926_RS17615	4172091..4172543	-	453	WP_160694229.1	DUF4348 domain-containing protein	-
GU926_RS17620	4172619..4172918	-	300	WP_160694231.1	HigA family addiction module antidote protein	Antitoxin
GU926_RS17625	4172924..4173205	-	282	WP_160694233.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GU926_RS17630	4173413..4173958	-	546	WP_160694235.1	hypothetical protein	-
GU926_RS17635	4174050..4174625	-	576	WP_160694236.1	SUKH-4 family immunity protein	-
GU926_RS17640	4174719..4175390	-	672	WP_160694238.1	hypothetical protein	-
GU926_RS17645	4175474..4175938	-	465	WP_160694240.1	hypothetical protein	-
GU926_RS17650	4176029..4176427	-	399	WP_160694242.1	hypothetical protein	-
GU926_RS17655	4177158..4177598	-	441	WP_160694244.1	hypothetical protein	-
GU926_RS17660	4177662..4178165	-	504	WP_160694246.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (4-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (21-68)

Sequences

Toxin

Protein

• Length: 94 a.a.
• Molecular weight: 10998.65 Da
• Isoelectric Point: 10.0914

>T148494 WP_160694233.1 NZ_CP047897:c4173205-4172924 [Nibribacter ruber]
MILSFGSKETEKIWTGERVAKLPMEIQTIGRRKLRMLHNSQNIADLRIPPSNRLEKLSGNLKEFYSIRINDQWRIIFQWN
NGQASEVEIVDYH

Nucleotide

• Length: 282 bp

>T148494 NZ_CP063748:88736-89218 [Klebsiella pneumoniae]
GTGGGACGTTTAACAGCACCAGAACCTTTGTCCGCTTTTCATCAGGTGGCTGAGTTCGTCAGCGGTGAGGCTGTGCTCGA
TGACTGGTTGAAGCAAAAGGGCCTCAAAAACCAGGCCCTCGGAGCTGCCAGAACATTTGTGGTGTGCAAGAAAGACACGA
AGCAAGTAGCCGGTTTTTACTCTCTGGCCACCGGCAGCGTCAACCATACAGAAGCGACAGGCAATCTTCGGCGTAACATG
CCAGATCCCATCCCTGTCATTATACTTGCCCGTCTTGCTGTCGATCTCTCATTCCATGGAAAAGGGCTTGGTGCTGATTT
ACTTCATGATGCAGTGCTTCGTTGCTATCGGGTTGCCGAGAATATTGGCGTACGTGCAATCATGGTTCATGCACTTACCG
AAGAAGCCAAAAATTTCTACATTCACCATGGTTTCAAACCATCACAAACTCAGCAGCGAACATTGTTTCTTAGACTCCCT
TAA

Antitoxin

Protein

• Length: 100 a.a.
• Molecular weight: 11589.24 Da
• Isoelectric Point: 6.4762

>AT148494 WP_160694231.1 NZ_CP047897:c4172918-4172619 [Nibribacter ruber]
MEKLPNIHPGEVLQEEFLIPLKITAYRLAKDISIPQTRVSEIIKGNRRITADTALRLSYYFGNSPKFWLGLQDDYDLEEE
KVNKQHELESIRRFEKNAA

Nucleotide

• Length: 300 bp

>AT148494 NZ_CP063748:88461-88748 [Klebsiella pneumoniae]
ATGCTATACAAGGGGTGTCTCATGAAATCAGATGTTCAACTTAACCTTAGAGCTAAGGAATCGCAGCGGGCGCTCATTGA
TGCAGCTGCGGAAATCCTTCACAAGTCACGTACAGATTTCATTCTGGAAATGGCCTGTCAGGCTGCCGAGAATGTGATCC
TTGACCGCCGTGTATTTAACTTTAACGATGAGCAATATGCGGAGTTCATCGACATGCTTGATGCACCTGTCGTAGATGAT
CCCGTTATCGAAAAACTGCTGGCAAGGAAACCTCAGTGGGACGTTTAA