Detailed information of TA system TA148480

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): hok-sok/-
• Location: 116273..116699
• Replicon: plasmid pLD22-1-135kb
• Accession: NZ_CP047878
• Organism: Escherichia coli strain LD22-1

Toxin (Protein)

• Gene name: hok
• Uniprot ID: P11895
• Locus tag: GUU87_RS24805
• Protein ID: WP_001312861.1
• Coordinates: 116273..116431 (-)
• Length: 53 a.a.

Antitoxin (RNA)

Gene name	sok
Locus tag	-
Coordinates	116475..116699 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU87_RS24770	111319..111546	-	228	WP_001254386.1	conjugal transfer relaxosome protein TraY	-
GUU87_RS24775	111640..112326	-	687	WP_000332484.1	PAS domain-containing protein	-
GUU87_RS24780	112517..112900	-	384	WP_000124981.1	relaxosome protein TraM	-
GUU87_RS24785	113177..113824	+	648	WP_000614936.1	transglycosylase SLT domain-containing protein	-
GUU87_RS24790	114121..114942	-	822	WP_001234469.1	DUF945 domain-containing protein	-
GUU87_RS24795	115064..115351	-	288	WP_000107535.1	hypothetical protein	-
GUU87_RS25135	115376..115582	-	207	WP_000547939.1	hypothetical protein	-
GUU87_RS24805	116273..116431	-	159	WP_001312861.1	type I toxin-antitoxin system Hok family toxin	Toxin
-	116475..116699	-	225	NuclAT_0	-	Antitoxin
-	116475..116699	-	225	NuclAT_0	-	Antitoxin
-	116475..116699	-	225	NuclAT_0	-	Antitoxin
-	116475..116699	-	225	NuclAT_0	-	Antitoxin
GUU87_RS24810	116511..116699	+	189	WP_001299721.1	hypothetical protein	-
GUU87_RS24815	116711..117430	-	720	WP_001276228.1	plasmid SOS inhibition protein A	-
GUU87_RS24820	117427..117861	-	435	WP_000845895.1	conjugation system SOS inhibitor PsiB	-
GUU87_RS24825	117916..119874	-	1959	WP_001145469.1	ParB/RepB/Spo0J family partition protein	-
GUU87_RS24830	119933..120166	-	234	WP_000006004.1	DUF905 domain-containing protein	-
GUU87_RS24835	120222..120749	-	528	WP_000290793.1	single-stranded DNA-binding protein	-
GUU87_RS24845	121222..121494	-	273	WP_072133095.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Conjugative plasmid	blaTEM-1C / dfrA14 / qnrS1 / tet(A) / floR	iroB / iroC / iroD / iroE / iroN	1..135123	135123

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Hok/gef cell toxic protein (8-48)

Sequences

Toxin

Protein

• Length: 53 a.a.
• Molecular weight: 6082.32 Da
• Isoelectric Point: 9.2584

>T148480 WP_001312861.1 NZ_CP047878:c116431-116273 [Escherichia coli]
MKLPRSSLVWCVLIVCLTLLIFTYLTRKSLCEIRYRDGHREVAAFMAYESGK

Nucleotide

• Length: 159 bp

>T148480 NZ_CP047878:c116431-116273 [Escherichia coli]
ATGAAACTACCACGAAGTTCCCTTGTCTGGTGTGTGTTGATCGTGTGTCTCACACTGTTGATATTCACTTATCTGACACG
AAAATCGCTGTGCGAGATTCGTTACAGAGACGGACACAGGGAGGTGGCGGCTTTCATGGCTTACGAATCCGGTAAGTAG

Antitoxin

Nucleotide

• Length: 225 bp

>AT148480 NZ_CP047878:c116699-116475 [Escherichia coli]
TCACACGGATTTCCCGTGAACGGTCTGAATGAGCGGATTATTTTCAGGGAAAGTGAGTGTGGTCAGCGTGCAGGTATATG
GGCTATGATGTGCCCGGCGCTTGAGGCTTTCTGCCTCATGACGTGAAGGTGGTTTGTTGCCGTGTTGTGTGGCAGAAAGA
AGATAGCCCCGTAGTAAGTTAATTTTCATTAACCACCACGAGGCATCCCTATGTCTAGTCCACAT

Structures

Toxin

• AlphaFold DB: P11895

Antitoxin