Detailed information of TA system TA148478

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HTH(antitoxin)
• Location: 4569366..4569968
• Replicon: chromosome
• Accession: NZ_CP047876
• Organism: Escherichia coli strain LD22-1

Toxin (Protein)

• Gene name: higB
• Uniprot ID: U9XIS6
• Locus tag: GUU87_RS21915
• Protein ID: WP_000897305.1
• Coordinates: 4569657..4569968 (-)
• Length: 104 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GUU87_RS21910
• Protein ID: WP_000356397.1
• Coordinates: 4569366..4569656 (-)
• Length: 97 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GUU87_RS21885	4565291..4566193	+	903	WP_000331377.1	formate dehydrogenase O subunit beta	-
GUU87_RS21890	4566190..4566825	+	636	WP_000829013.1	formate dehydrogenase cytochrome b556 subunit	-
GUU87_RS21895	4566822..4567751	+	930	WP_000027708.1	formate dehydrogenase accessory protein FdhE	-
GUU87_RS21900	4568081..4568323	-	243	WP_001087409.1	hypothetical protein	-
GUU87_RS21905	4568543..4568761	-	219	WP_001315930.1	ribbon-helix-helix domain-containing protein	-
GUU87_RS21910	4569366..4569656	-	291	WP_000356397.1	helix-turn-helix domain-containing protein	Antitoxin
GUU87_RS21915	4569657..4569968	-	312	WP_000897305.1	hypothetical protein	Toxin
GUU87_RS21920	4570197..4571105	+	909	WP_001162704.1	alpha/beta hydrolase	-
GUU87_RS21925	4571169..4572110	-	942	WP_001297068.1	fatty acid biosynthesis protein FabY	-
GUU87_RS21930	4572155..4572592	-	438	WP_000560983.1	D-tyrosyl-tRNA(Tyr) deacylase	-
GUU87_RS21935	4572589..4573461	-	873	WP_000920762.1	virulence factor BrkB family protein	-
GUU87_RS21940	4573455..4574054	-	600	WP_001295269.1	glucose-1-phosphatase	-
GUU87_RS21945	4574153..4574938	-	786	WP_000059678.1	DeoR/GlpR family DNA-binding transcription regulator	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

No domain identified.

Antitoxin

• Cro/C1-type helix-turn-helix domain (36-85)

Sequences

Toxin

Protein

• Length: 104 a.a.
• Molecular weight: 12190.19 Da
• Isoelectric Point: 9.7791

>T148478 WP_000897305.1 NZ_CP047876:c4569968-4569657 [Escherichia coli]
MLFIETEIFTEDVQKLLTDDEFSRFQFFLALNPDYGEVIPETGGLRKVRWVSGGKGKRAGVRVIYFHQVKHYEIRLLLIY
RKGIKDDLSPQEKAMLRLLNTRW

Nucleotide

• Length: 312 bp

>T148478 NZ_CP063745:1150280-1150468 [Staphylococcus aureus]
ATGTCTTTACATTTTGCAATTCTGTTTTGGCTAGCATTAATTTTCTTAGTTGCCGCTACGTTTATACTCGTATTAATGAA
AAAAACTGGCAAAGAATCTAAAAAAGAGTCCTATTTAAGTTTCACTGTCATTCTCTATATTTTTGGATTCGCTATATTAA
TATACACATTTATATTTGGTGTGCTATAA

Antitoxin

Protein

• Length: 97 a.a.
• Molecular weight: 10846.43 Da
• Isoelectric Point: 10.8907

>AT148478 WP_000356397.1 NZ_CP047876:c4569656-4569366 [Escherichia coli]
MDKALFERLTHSMAQMNEIIEGTRQPSRTFEVDAMKIKEIRRASGLSQSKFADLISVSVDTLRNWEQGRRSPTGPAKALL
RAIANDPQHVLQALNR

Nucleotide

• Length: 291 bp

>AT148478 NZ_CP063745:1149992-1150252 [Staphylococcus aureus]
ATGAATAACATTTTGTTAAATGCTATCAATATAGTTATTACTACCACTTTTGTTATCTTTAATATTTTAATCACATATAA
TAAAGATTTAGATGATTTATGTTGGCTCCTGCCTGGTATTATCATTTGTGGTGTGATACTCATAGTATCCTTTACCATTG
CAATGATAACTAAAAACTGGTTAAGTGAAATATTATTTTTTATAAATATCGTACTCGTTCTCTATTACATTTATCCTATT
TTTTATAGTTTTATAGGTTAA

Structures

Toxin

• AlphaFold DB: A0A0E0Y7I7