Detailed information of TA system TA148447

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2405547..2406064
• Replicon: chromosome
• Accession: NZ_CP047867
• Organism: Staphylococcus aureus strain UP_419

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: E3S59_RS11765
• Protein ID: WP_000113265.1
• Coordinates: 2405798..2406064 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3S59_RS11760
• Protein ID: WP_000584499.1
• Coordinates: 2405547..2405798 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S59_RS11730	2401245..2401964	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3S59_RS11735	2401961..2402692	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3S59_RS11740	2402789..2404039	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
E3S59_RS11745	2404294..2404656	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3S59_RS11750	2404675..2405274	+	600	WP_000162810.1	DsbA family protein	-
E3S59_RS11755	2405281..2405382	+	102	Protein_2275	hypothetical protein	-
E3S59_RS11760	2405547..2405798	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3S59_RS11765	2405798..2406064	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
E3S59_RS11770	2406099..2406170	+	72	Protein_2278	hypothetical protein	-
E3S59_RS11775	2406359..2407906	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3S59_RS11780	2408092..2408559	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
E3S59_RS11785	2408643..2408825	+	183	WP_000230292.1	hypothetical protein	-
E3S59_RS11790	2409042..2409866	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
E3S59_RS11795	2410100..2410636	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T148447 WP_000113265.1 NZ_CP047867:2405798-2406064 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148447 NZ_CP063518:c3313955-3313557 [Escherichia coli]
ATGCTGAAATTCATGCTGGATACCAATATCTGTATTTTCACTATTAAGAATAAACCAGTACATGTCAGAGAGCGGTTTAA
CCTGAATTCTGGCCGGATGTGTATAAGTTCTGTAACATTGATGGAGCTTATCTATGGGGCAGAAAAAAGTCAGATGCCAG
AACGCAATTTGGCGGTTATTGAAGGTTTCGTATCTCGTTTGGTAGTGCTTGATTATGATAGTGCTGCTGCGACGCATACC
GGGCAAATCAGGGCCGAGCTTGCCCGGCAGGGAAGGCCTGTTGGACCTTTTGACCAGATGATAGCGGGACATGCTCGCAG
TCGCGGTTTGATTGTGGTTACCAACAATACCCGAGAGTTTGAGCGAGTGGCGGGAATCAGACTAGAAGACTGGAGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148447 WP_000584499.1 NZ_CP047867:2405547-2405798 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148447 NZ_CP063518:c3314182-3313955 [Escherichia coli]
ATGGAAACTACTGTATTTTTAAGTAATCGCAGTCAGGCCGTCAGATTACCGAAAGCGGTCGCTCTGCCAGAAGACGTTAA
GAAAGTAGAAATTATTGCGATTGGTCGTACAAGAATCATCACTCCCGCCGGTGAGTCCTGGGACAGTTGGTTTGATGGCG
AAAATGTGAGTGCGGATTTTATGGATATCCGTGATCAACCCGCAATGCAGGAAAGGGAATCATTTTGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60