Detailed information of TA system TA148413

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2405185..2405702
• Replicon: chromosome
• Accession: NZ_CP047863
• Organism: Staphylococcus aureus strain UP_1106

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: E3S63_RS11825
• Protein ID: WP_000113265.1
• Coordinates: 2405436..2405702 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3S63_RS11820
• Protein ID: WP_000584499.1
• Coordinates: 2405185..2405436 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S63_RS11790	2400883..2401602	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3S63_RS11795	2401599..2402330	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3S63_RS11800	2402427..2403677	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
E3S63_RS11805	2403932..2404294	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3S63_RS11810	2404313..2404912	+	600	WP_000162810.1	DsbA family protein	-
E3S63_RS11815	2404919..2405020	+	102	Protein_2288	hypothetical protein	-
E3S63_RS11820	2405185..2405436	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3S63_RS11825	2405436..2405702	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
E3S63_RS11830	2405737..2405808	+	72	Protein_2291	hypothetical protein	-
E3S63_RS11835	2405997..2407544	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3S63_RS11840	2407730..2408197	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
E3S63_RS11845	2408281..2408463	+	183	WP_000230292.1	hypothetical protein	-
E3S63_RS11850	2408680..2409504	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
E3S63_RS11855	2409738..2410274	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T148413 WP_000113265.1 NZ_CP047863:2405436-2405702 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148413 NZ_CP063515:c2276123-2275809 [Escherichia coli]
ATGCACCTGATAACTCAAAAAGCATTGAAAGATGCTGCGGAAAAATACCCGCAACATAAAACGGAGTTGGTGGCTCTGGG
GAACACGATTGCTAAGGGATATTTCAAAAAACCTGAGTCATTAAAAGCAGTATTCCCTTCTCTGGATAACTTCAAATATC
TGGATAAGCATTATGTTTTCAATGTCGGGGGCAATGAATTACGTGTTGTAGCAATGGTCTTTTTTGAATCGCAAAAGTGC
TACATACGTGAAGTTATGACGCATAAAGAATACGATTTCTTTACCGCCGTTCATCGTACTAAGGGGAAAAAATGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148413 WP_000584499.1 NZ_CP047863:2405185-2405436 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148413 NZ_CP063515:c2275812-2275396 [Escherichia coli]
ATGATTGCGATTGCCGACATCTTGCAAGCAGGAGAAAAGCTAACTGCTGTGGCACCTTTTCTGGCGGGTATTCAGAACGA
GGAACAATACACCCAGGCGCTGGAACTGGTAGATCATCTGCTGCTCAACGATCCTGAAAACCCCTTACTGGATCTGGTGT
GTGCCAAAATAACCGCGTGGGAAGAATCAGCGCCCGAATTTGTGGAATTTAATGCCATGGCTCAAGCCATGCCTGGCGGT
ATAGCCGTGATTCGTACCCTTATGGATCAATATGGTTTAACCCTTTCCGATCTTCCGGAAATAGGCAGTAAATCGATGGT
GTCACGCGTTTTGAGCGGGAAAAGGAAATTAACGCTGGAACACGCTAAAAAACTGGCAACGCGATTCGGTATTTCTCCCG
CCTTGTTTATTGATTAA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60