Detailed information of TA system TA148396

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2439494..2440011
• Replicon: chromosome
• Accession: NZ_CP047861
• Organism: Staphylococcus aureus strain UP_1322

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: E3S64_RS12055
• Protein ID: WP_000113265.1
• Coordinates: 2439745..2440011 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3S64_RS12050
• Protein ID: WP_000584499.1
• Coordinates: 2439494..2439745 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S64_RS12020	2435192..2435911	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3S64_RS12025	2435908..2436639	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3S64_RS12030	2436736..2437986	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
E3S64_RS12035	2438241..2438603	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3S64_RS12040	2438622..2439221	+	600	WP_000162810.1	DsbA family protein	-
E3S64_RS12045	2439228..2439329	+	102	Protein_2335	hypothetical protein	-
E3S64_RS12050	2439494..2439745	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3S64_RS12055	2439745..2440011	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
E3S64_RS12060	2440046..2440117	+	72	Protein_2338	hypothetical protein	-
E3S64_RS12065	2440306..2441853	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3S64_RS12070	2442039..2442506	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
E3S64_RS12075	2442590..2442772	+	183	WP_000230292.1	hypothetical protein	-
E3S64_RS12080	2442989..2443813	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
E3S64_RS12085	2444047..2444583	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T148396 WP_000113265.1 NZ_CP047861:2439745-2440011 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148396 NZ_CP063511:2947193-2947444 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTCATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATACAACTCGCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATTATTGAGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148396 WP_000584499.1 NZ_CP047861:2439494-2439745 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148396 NZ_CP063511:2947441-2947695 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60