Detailed information of TA system TA148337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 1733995..1734418
• Replicon: chromosome
• Accession: NZ_CP047854
• Organism: Staphylococcus aureus strain UP_1525

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: E3S68_RS08785
• Protein ID: WP_031766094.1
• Coordinates: 1734167..1734418 (-)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E3S68_RS08780
• Protein ID: WP_000028421.1
• Coordinates: 1733995..1734177 (-)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S68_RS08735	1729295..1729585	-	291	WP_000665204.1	VRR-NUC domain-containing protein	-
E3S68_RS08740	1729719..1729823	+	105	WP_015978074.1	hypothetical protein	-
E3S68_RS14495	1729926..1732373	-	2448	WP_031765980.1	virulence-associated E family protein	-
E3S68_RS08750	1732425..1732625	-	201	WP_000265258.1	DUF1514 family protein	-
E3S68_RS08755	1732693..1732845	-	153	WP_031767608.1	transcriptional activator RinB	-
E3S68_RS08760	1732838..1733041	-	204	WP_001072794.1	hypothetical protein	-
E3S68_RS08765	1733038..1733232	-	195	WP_000132920.1	hypothetical protein	-
E3S68_RS08770	1733229..1733435	-	207	WP_000195785.1	DUF1381 domain-containing protein	-
E3S68_RS08775	1733472..1734002	-	531	WP_000185651.1	dUTP pyrophosphatase	-
E3S68_RS08780	1733995..1734177	-	183	WP_000028421.1	hypothetical protein	Antitoxin
E3S68_RS08785	1734167..1734418	-	252	WP_031766094.1	DUF1024 family protein	Toxin
E3S68_RS08790	1734411..1734719	-	309	WP_031895322.1	hypothetical protein	-
E3S68_RS08795	1734716..1735063	-	348	WP_000979209.1	hypothetical protein	-
E3S68_RS08800	1735128..1735376	-	249	WP_111023884.1	phi PVL orf 51-like protein	-
E3S68_RS08805	1735376..1735630	-	255	WP_000111487.1	DUF3310 domain-containing protein	-
E3S68_RS08810	1735630..1736029	-	400	Protein_1693	PVL family protein	-
E3S68_RS08815	1736029..1736214	-	186	WP_001164629.1	DUF3113 family protein	-
E3S68_RS08820	1736227..1738179	-	1953	WP_001796208.1	DNA polymerase	-
E3S68_RS08825	1738248..1738805	-	558	WP_000645042.1	DUF2815 family protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	geh	1701900..1751644	49744

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9393.31 Da
• Isoelectric Point: 4.0362

>T148337 WP_031766094.1 NZ_CP047854:c1734418-1734167 [Staphylococcus aureus]
MNNREKIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGIELDEAVGIMTGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T148337 NZ_CP063499:609419-609670 [Escherichia coli]
ATGCGTACAATTAGCTACAGCGAAGCGCGTCAGAATTTGTCGGCAACAATGATGAAAGCCGTTGAAGATCATGCCCCGAT
CCTTATTACTCGTCAGAATGGAGAGGCTTGTGTTCTGATGTCACTCGAAGAATATAACTCCCTGGAAGAGACGGCTTATC
TACTGCGTTCCCCCGCTAACGCCCGGAGATTGATGGACTCAATCGATAGCCTGAAATCAGGCAAAGGAACGGAAAAGGAC
ATCATTGAGTGA

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6683.59 Da
• Isoelectric Point: 4.3791

>AT148337 WP_000028421.1 NZ_CP047854:c1734177-1733995 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEAQEND

Nucleotide

• Length: 183 bp

>AT148337 NZ_CP063499:609667-609921 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGACGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACGCCATTTGAAGGTAAGGGGAAGCCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACCGACGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA