Detailed information of TA system TA148323

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2353725..2354242
• Replicon: chromosome
• Accession: NZ_CP047852
• Organism: Staphylococcus aureus strain UP_274

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: E3S71_RS11550
• Protein ID: WP_000113262.1
• Coordinates: 2353976..2354242 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3S71_RS11545
• Protein ID: WP_000584499.1
• Coordinates: 2353725..2353976 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S71_RS11515	2349423..2350142	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3S71_RS11520	2350139..2350870	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3S71_RS11525	2350967..2352217	-	1251	WP_001012227.1	FemA/FemB family glycyltransferase FmhA	-
E3S71_RS11530	2352472..2352834	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3S71_RS11535	2352853..2353452	+	600	WP_000162813.1	DsbA family protein	-
E3S71_RS11540	2353459..2353560	+	102	WP_001791744.1	hypothetical protein	-
E3S71_RS11545	2353725..2353976	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3S71_RS11550	2353976..2354242	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
E3S71_RS11555	2354277..2354348	+	72	Protein_2224	hypothetical protein	-
E3S71_RS11560	2354537..2356087	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3S71_RS11565	2356273..2356740	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
E3S71_RS11570	2356824..2357006	+	183	WP_000230294.1	hypothetical protein	-
E3S71_RS11575	2357223..2358047	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
E3S71_RS11580	2358280..2358816	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T148323 WP_000113262.1 NZ_CP047852:2353976-2354242 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148323 NZ_CP063492:c5080288-5079992 [Escherichia coli]
ATGGAAAAACGCACACCACATACACGTTTGAGTCAGGTTAAAAAACTTGTCAATGCCGGGCAAGTTCGTACAACACGTAG
TGCCCTGTTAAATGCAGATGAGTTAGGTTTGGATTTTGATGGTATGTGTAATGTTATCATTGGATTATCAGAGAGCGACT
TTTATAAAAGCATGACCACCTACTCTGATCATACTATCTGGCAGGATGTTTACAGACCCAGGCTTGTTACAGGCCAGGTT
TATCTTAAAATTACGGTAATTCATGACGTACTGATCGTCTCGTTTAAGGAGAAGTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148323 WP_000584499.1 NZ_CP047852:2353725-2353976 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148323 NZ_CP063492:c5079990-5079595 [Escherichia coli]
ATGAAATGTCCGGTTTGCCACCAGGGAGAAATGGTTTCTGGCATTAAAGATATTCCATACACCTTCCGTGGACGAAAAAC
AGTATTGAAAGGTATCCACGGTTTATATTGTGTCCATTGCGAAGAGAGCATCATGAATAAAGAAGAGTCAGATGCTTTCA
TGGCGCAAGTAAAGGCATTTCGGGCTTCGGTGAATGCCGAAACAGTGGCACCTGAATTTATAGTGAAGGTTCGAAAAAAG
CTCTCTCTTACCCAAAAAGAGGCAAGCGAAATTTTTGGGGGAGGTGTAAATGCGTTTTCGCGTTACGAAAAAGGCAATGC
CCAACCTCATCCTTCCACAATCAAACTTTTACGTGTTCTGGATAAGCATCCAGAACTATTGAATGAAATCCGTTAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60