Detailed information of TA system TA148313

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 19314..19652
• Replicon: chromosome
• Accession: NZ_CP047852
• Organism: Staphylococcus aureus strain UP_274

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: E3S71_RS00100
• Protein ID: WP_011447039.1
• Coordinates: 19314..19490 (+)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	19478..19652 (-)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S71_RS00080	14552..18334	+	3783	WP_160199939.1	hypothetical protein	-
E3S71_RS00085	18324..18476	+	153	WP_001153681.1	hypothetical protein	-
E3S71_RS00090	18523..18810	+	288	WP_001040261.1	hypothetical protein	-
E3S71_RS00095	18868..19164	+	297	WP_000539688.1	DUF2951 domain-containing protein	-
E3S71_RS00100	19314..19490	+	177	WP_011447039.1	putative holin-like toxin	Toxin
-	19478..19652	-	175	-	-	Antitoxin
E3S71_RS00110	19702..19956	+	255	WP_000611512.1	phage holin	-
E3S71_RS00115	19968..20723	+	756	WP_000861038.1	CHAP domain-containing protein	-
E3S71_RS00120	20914..21405	+	492	WP_000920037.1	staphylokinase	-
E3S71_RS00125	22055..22390	+	336	Protein_24	SH3 domain-containing protein	-
E3S71_RS00130	22485..22934	-	450	WP_000727643.1	chemotaxis-inhibiting protein CHIPS	-
E3S71_RS00135	23616..23966	+	351	WP_000702262.1	complement inhibitor SCIN-A	-
E3S71_RS00140	24019..24279	-	261	WP_001791826.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	sak / chp / scn	52..23966	23914

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T148313 WP_011447039.1 NZ_CP047852:19314-19490 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T148313 NZ_CP063492:c2723421-2722927 [Escherichia coli]
ATGATTCAGGCCCCCGAGCCACTCATGGCAAGGCATCAATTCACCTCTTTTTGCTCGGGAGTAGAAACGATGGATAACTG
GCTAAAGCAGAGAGCCCTAAAAAATCAGCTTGCCGGCGCATCACGTACATTTGTTAGCTGTGATACGTATAGCAACGTTT
TGGCATATTATTCGCTTGCTTCCAGCGCCGTTGAAACGTATGTCGCGACAGGACGATTTCGCCGAAATATGCCAGAACCG
ATCCCTGTTGTAGTACTTGGTCGTCTGGCAATAGATAAATCATTACAGGGTCAGGGCATCGGTCGTGCGATGGTCAGAGA
TGCCGGTCTACGAGTACTACAGGCGGCTGAAGTCATTGGTATTCGCGGAATGTTGGTCCATGCGTTATCAGACCAGGCCC
GTGAGTTTTATCTGCGAGTGGGGTTTGAACCATCGCCTGTCGATTCTATGATACTGATGGCAACATTGGCTGATTTGCAG
GAATGTCTTAAATAG

Antitoxin

Nucleotide

• Length: 175 bp

>AT148313 NZ_CP047852:c19652-19478 [Staphylococcus aureus]
ATTTGATATTTATATTATGGTGTGTTAATTTATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCC
GTTAAAAAGACGGTGGCTATTTTAGATTAAAGATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGAT
GGTTATTTTTTATTG

Structures

Toxin

• PDB: 7NS1

Antitoxin