Detailed information of TA system TA148290

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2332376..2332893
• Replicon: chromosome
• Accession: NZ_CP047849
• Organism: Staphylococcus aureus strain UP_403

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: E3S76_RS11390
• Protein ID: WP_000113262.1
• Coordinates: 2332627..2332893 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3S76_RS11385
• Protein ID: WP_000584499.1
• Coordinates: 2332376..2332627 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S76_RS11355	2328074..2328793	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3S76_RS11360	2328790..2329521	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3S76_RS11365	2329618..2330868	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
E3S76_RS11370	2331123..2331485	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3S76_RS11375	2331504..2332103	+	600	WP_000162813.1	DsbA family protein	-
E3S76_RS11380	2332110..2332211	+	102	WP_001791744.1	hypothetical protein	-
E3S76_RS11385	2332376..2332627	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3S76_RS11390	2332627..2332893	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
E3S76_RS11395	2332928..2332999	+	72	Protein_2203	hypothetical protein	-
E3S76_RS11400	2333188..2334735	+	1548	WP_001551849.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3S76_RS11405	2334921..2335388	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
E3S76_RS11410	2335472..2335654	+	183	WP_000230294.1	hypothetical protein	-
E3S76_RS11415	2335871..2336695	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
E3S76_RS11420	2336928..2337464	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T148290 WP_000113262.1 NZ_CP047849:2332627-2332893 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148290 NZ_CP063487:2806648-2806983 [Escherichia coli]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTGTACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148290 WP_000584499.1 NZ_CP047849:2332376-2332627 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148290 NZ_CP063487:2806400-2806648 [Escherichia coli]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60