Detailed information of TA system TA148273

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2361241..2361758
• Replicon: chromosome
• Accession: NZ_CP047847
• Organism: Staphylococcus aureus strain UP_522

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: E3S80_RS11765
• Protein ID: WP_000113262.1
• Coordinates: 2361492..2361758 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3S80_RS11760
• Protein ID: WP_000584499.1
• Coordinates: 2361241..2361492 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S80_RS11730	2356939..2357658	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3S80_RS11735	2357655..2358386	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3S80_RS11740	2358483..2359733	-	1251	WP_001012223.1	FemA/FemB family glycyltransferase FmhA	-
E3S80_RS11745	2359988..2360350	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3S80_RS11750	2360369..2360968	+	600	WP_000162813.1	DsbA family protein	-
E3S80_RS11755	2360975..2361076	+	102	WP_031774922.1	hypothetical protein	-
E3S80_RS11760	2361241..2361492	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3S80_RS11765	2361492..2361758	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
E3S80_RS11770	2361793..2361864	+	72	Protein_2281	hypothetical protein	-
E3S80_RS11775	2362053..2363600	+	1548	WP_000727756.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3S80_RS11780	2363786..2364253	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
E3S80_RS11785	2364337..2364519	+	183	WP_000230294.1	hypothetical protein	-
E3S80_RS11790	2364736..2365560	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
E3S80_RS11795	2365793..2366329	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T148273 WP_000113262.1 NZ_CP047847:2361492-2361758 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148273 NZ_CP063483:c4368905-4368507 [Escherichia coli]
ATGCGGGTATTCAAAACAAAACTTATTCGCCTGCAACTTACAGCAGAGGAACTTGATGCGTTAACGGCGGATTTTATTTC
CTATAAGCGTGACGGTGTTTTGCCAGATATATTTGGTCGCGATGCACTCTACGACGACTCCTTTACCTGGCCATTAATCA
AATTTGAGCGAGTTGCTCATATTCATCTGGCAAATGAGAATAATCCATTTCCGCCACAGTTGCGCCAATTCAGCAGAACG
AATGACGAAGCGCATTTGGTATATTGTCAGGGGGCGTTTGATGAGCAAGCATGGTTGCTCATTGCCATTCTGAAACCTGA
ACCTCATAAACTGGCTCGAGATAACAACCAAATGCATAAAATTGGGAAAATGGCAGAAGCGTTTCGCATGCGTTTTTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148273 WP_000584499.1 NZ_CP047847:2361241-2361492 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148273 NZ_CP063483:c4369201-4368908 [Escherichia coli]
ATGCATCGAATTCTCGCTGAAAAATCGGTCAATATCACTGAGTTACGTAAAAACCCAGCTAAATACTTTATTGATCAACC
GGTTGCGGTTCTTTCTAATAATCGCCCCGCAGGATATCTCTTAAGTGCCAGCGCATTCGAAGCGTTAATGGACATGCTTG
CTGAACAAGAGGAGAAAAAGCCCATAAAGGCGCGCTTCCGTCCAAGTGCTGCAAGATTAGAGGAAATTACACGCCGCGCT
GAACAATATCTTAATGATATGACGGATGATGATTTCAATGACTTTAAGGAATAA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60