Detailed information of TA system TA148121

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2399906..2400423
• Replicon: chromosome
• Accession: NZ_CP047822
• Organism: Staphylococcus aureus strain UP_1278

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: E3T02_RS11785
• Protein ID: WP_000113262.1
• Coordinates: 2400157..2400423 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3T02_RS11780
• Protein ID: WP_000584499.1
• Coordinates: 2399906..2400157 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T02_RS11745	2394925..2395704	+	780	WP_017804733.1	transporter substrate-binding domain-containing protein	-
E3T02_RS11750	2395685..2396404	+	720	WP_160194443.1	amino acid ABC transporter permease	-
E3T02_RS11755	2396401..2397132	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3T02_RS11760	2397229..2398398	-	1170	WP_160194444.1	aminoacyltransferase	-
E3T02_RS11765	2398653..2399015	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3T02_RS11770	2399034..2399633	+	600	WP_000162813.1	DsbA family protein	-
E3T02_RS11775	2399640..2399741	+	102	WP_001791744.1	hypothetical protein	-
E3T02_RS11780	2399906..2400157	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3T02_RS11785	2400157..2400423	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
E3T02_RS11790	2400458..2400529	+	72	Protein_2278	hypothetical protein	-
E3T02_RS11795	2400718..2402268	+	1551	WP_000727763.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3T02_RS11800	2402454..2402921	+	468	WP_000153346.1	SRPBCC domain-containing protein	-
E3T02_RS11805	2403015..2404187	-	1173	WP_000195429.1	IS256-like element IS256 family transposase	-
E3T02_RS11810	2404340..2404522	+	183	WP_000079142.1	hypothetical protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	2403015..2404187	1172

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T148121 WP_000113262.1 NZ_CP047822:2400157-2400423 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148121 NZ_CP063454:c4043428-4043132 [Pseudomonas putida]
ATGGAAAAGAGAACGCCTCATTGCCCGCTGGAGCGGGTAAGAGCACTGGTGGGCGCTGGCCGCATCAGCCCTACAACCGC
TTCCTTACGAGGCGCTCAGGCCTTGGGAATGGAATATGCGGACATGCTGGAGGTCATCACCGGTCTGGAGCGCAGAGATT
TCTACAAAAGCATGACCAGTTATGCCGACCACCGCGTTTGGCAAGATGTCTATCGGCCGCTTACGGTAAAGGGATATCTG
TACCTGAAACTGTCAGTGGTAGATGACGCGCTCATCGTGTCTTTCAAGGAGTTGTGA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148121 WP_000584499.1 NZ_CP047822:2399906-2400157 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148121 NZ_CP063454:c4043129-4042728 [Pseudomonas putida]
ATGAGATGCCCAATTTGCGGAGGTGCCGAGCTCGCGCTAGACACTCAGGACATGCCCTACCGGTATAAGGGGGAATCGAC
CCTGATACCTGATGTGACGGGCGATTACTGTTCTGTCTGTGGTGAGGCAGTGCTAAGTCATGACGAGGCTATGCGAGTCA
GCGATCTGATGTCTGCCTTCAATCGCCAAGTCAATGCTCTAGACGTTGATCCCGATTTCATCGTTTCCGTACGCAAGAAA
TTTGATCTGGATCAGCGGGAGGCTGGGGAAATATTCGGTGGAGGAGTCAATGCTTTTTCACGTTATGAGAATGGCAAAAC
CAAACCGCCGGTGGCGCTGATCAAATTGTTCAAGTTGCTCGACCGTCACCCGGAGCTCTTTGAGGAAGTCAGGACTGCGT
GA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7BWF
• PDB: 6L8E
• AlphaFold DB: T1YB60