Detailed information of TA system TA148106

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2342304..2342821
• Replicon: chromosome
• Accession: NZ_CP047820
• Organism: Staphylococcus aureus strain UP_1395

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YC79
• Locus tag: E3T05_RS11470
• Protein ID: WP_000113262.1
• Coordinates: 2342555..2342821 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3T05_RS11465
• Protein ID: WP_000584499.1
• Coordinates: 2342304..2342555 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T05_RS11435	2338002..2338721	+	720	WP_015581909.1	amino acid ABC transporter permease	-
E3T05_RS11440	2338718..2339449	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3T05_RS11445	2339546..2340796	-	1251	WP_001012222.1	FemA/FemB family glycyltransferase FmhA	-
E3T05_RS11450	2341051..2341413	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3T05_RS11455	2341432..2342031	+	600	WP_000162813.1	DsbA family protein	-
E3T05_RS11460	2342038..2342139	+	102	WP_001791744.1	hypothetical protein	-
E3T05_RS11465	2342304..2342555	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3T05_RS11470	2342555..2342821	+	267	WP_000113262.1	Txe/YoeB family addiction module toxin	Toxin
E3T05_RS11475	2342856..2342927	+	72	Protein_2216	hypothetical protein	-
E3T05_RS11480	2343116..2344666	+	1551	WP_000727762.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3T05_RS11485	2344852..2345319	+	468	WP_000153344.1	SRPBCC domain-containing protein	-
E3T05_RS11490	2345403..2345585	+	183	WP_000230294.1	hypothetical protein	-
E3T05_RS11495	2345802..2346626	+	825	WP_000572040.1	formate/nitrite transporter family protein	-
E3T05_RS11500	2346859..2347395	+	537	WP_015581908.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (5-88)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10437.95 Da
• Isoelectric Point: 9.9143

>T148106 WP_000113262.1 NZ_CP047820:2342555-2342821 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNDKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148106 NZ_CP063441:746776-747186 [Enterobacter asburiae]
GTGGTCCTGTGGCAATCTGATCTTCGCGTCTCGTGGCGCTCACAGTGGATGTCTTTATTGCTCCACGGCCTGGTTGCGGC
CTTTGTGTTGCTGATGCCGTGGCCGCTGAGCTATACCCCGCTGTGGCTGTTGTTACTCTCGTTTGTCGTGTTTGACAGCG
TACGCAGCCAGCGTCGCATTAATGCCCGTCAGGGAGAGATCAAGCTACTGATGGATTCCCGCCTGCGCTGGCAGGGTAAA
GAGTGGGATATTCTTGGTATGCCCTGGATGCTCAACTGCGGCATGATGTTACGGTTACGCAAGGTGGACGGCGGGCGTTG
CCAGCATCTGTGGCTGGCGGCTGACAGTATGGATGCTGCCGAGTGGCGAGACCTGCGCCGGATGCTGTTGCAACAGACGA
CGCAGGGATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148106 WP_000584499.1 NZ_CP047820:2342304-2342555 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148106 NZ_CP063441:746529-746795 [Enterobacter asburiae]
ATGGATATTAACAACAAGGCCCGTATTCACTGGGCGTGCCGTCGCGGCATGCGTGAACTTGATATTTCCATCATGCCTTT
CTTCGAATATGAGTATGACAGCTTAAGCGATGATGATAAGCGTCTGTTTGTTCGCCTGCTTGAGTCTGACGATCCGGATT
TATTCAACTGGCTGATGAACCACGGCAAACCCGCCGACACCGAATTGCAACGGATGGTGCAATTAATTCAAACACGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Toxin

• PDB: 6L8G
• PDB: 6L8F
• PDB: 7CUA
• PDB: 6L8E

Antitoxin

• AlphaFold DB: T1YB60