Detailed information of TA system TA148089

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2327162..2327679
• Replicon: chromosome
• Accession: NZ_CP047817
• Organism: Staphylococcus aureus strain UP_1433

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: E3T06_RS11335
• Protein ID: WP_000113265.1
• Coordinates: 2327413..2327679 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3T06_RS11330
• Protein ID: WP_000584499.1
• Coordinates: 2327162..2327413 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T06_RS11300	2322860..2323579	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3T06_RS11305	2323576..2324307	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3T06_RS11310	2324404..2325654	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
E3T06_RS11315	2325909..2326271	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3T06_RS11320	2326290..2326889	+	600	WP_000162810.1	DsbA family protein	-
E3T06_RS11325	2326896..2326997	+	102	Protein_2186	hypothetical protein	-
E3T06_RS11330	2327162..2327413	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3T06_RS11335	2327413..2327679	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
E3T06_RS11340	2327714..2327785	+	72	Protein_2189	hypothetical protein	-
E3T06_RS11345	2327974..2329521	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3T06_RS11350	2329707..2330174	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
E3T06_RS11355	2330258..2330440	+	183	WP_000230292.1	hypothetical protein	-
E3T06_RS11360	2330657..2331481	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
E3T06_RS11365	2331715..2332251	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T148089 WP_000113265.1 NZ_CP047817:2327413-2327679 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T148089 NZ_CP063426:33013-33429 [Kosakonia pseudosacchari]
GTGAACAGAACCTATATGTTGGACACGAACATCTGCTCATTCATCATGCGCGAGCAGCCGGAGGCGGTACTGAAGCGCCT
GGAGCAGGCGGTATTGCGCGGTCAGCGCATCGTGGTCTCGGCCATCACCTACTCCGAGATGCGCTTCGGCGCCACCGGAC
CCAAGGCCTCGCCGCGACACGTGCTGCTGGTTGACGCGTTCTGTGCCCGCCTCGATGCCGTCCTGCCCTGGGACCGTGCA
GCGGTGGATGCGACCACGGATATAAAGGTAGCGCTGCGCCTCGCCGGGACGCCGATAGGCCCGAACGACACGGCGATCGC
CGGGCACGCCATCGCCGCCGGCGCCGTGCTGGTTACCAATAATACGCGGGAATTTGAGCGGGTACCGGGTCTGGTACTCG
AAGACTGGGTGAAATAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT148089 WP_000584499.1 NZ_CP047817:2327162-2327413 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT148089 NZ_CP063426:32786-33016 [Kosakonia pseudosacchari]
ATGAGAACTGTATCCATTTTCAAAAATGGCAACAACCGCGCCATCCGCCTGCCCCGCGATCTGGATTTTGAAGGGGTGAG
CGAGCTGGAGATCGTCCGAGAAGGGGACAGCATCATCCTGCGTCCCGTCCGGCCGACCTGGGGGTCGTTCACGCAGTTGG
AGAAGGCCGATCCGGACTTTATGGCGGAGCGCGAGGACGTTGTCAGCGACGAAGGACGATTTAACCTGTGA

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60