Detailed information of TA system TA148015

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2215088..2215611
• Replicon: chromosome
• Accession: NZ_CP047807
• Organism: Staphylococcus aureus strain UP_1612

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: T1YCD0
• Locus tag: E3T15_RS10865
• Protein ID: WP_000074043.1
• Coordinates: 2215345..2215611 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YBB2
• Locus tag: E3T15_RS10860
• Protein ID: WP_000587618.1
• Coordinates: 2215088..2215345 (+)
• Length: 86 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T15_RS10840	2210161..2211354	+	1194	WP_000675407.1	MFS transporter	-
E3T15_RS10845	2211585..2212334	-	750	WP_000072466.1	hypothetical protein	-
E3T15_RS10850	2212529..2214106	+	1578	WP_000143503.1	FMN-binding glutamate synthase family protein	-
E3T15_RS10855	2214274..2214729	-	456	WP_000732076.1	DUF1307 domain-containing protein	-
E3T15_RS10860	2215088..2215345	+	258	WP_000587618.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3T15_RS10865	2215345..2215611	+	267	WP_000074043.1	Txe/YoeB family addiction module toxin	Toxin
E3T15_RS10870	2215967..2217043	+	1077	WP_001076673.1	M42 family metallopeptidase	-
E3T15_RS10875	2217291..2217953	+	663	WP_000923524.1	ATP-binding cassette domain-containing protein	-
E3T15_RS10880	2217946..2218722	+	777	WP_000072147.1	iron export ABC transporter permease subunit FetB	-
E3T15_RS10885	2219118..2220305	+	1188	WP_000026194.1	MFS transporter	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin YoeB (4-87)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-68)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10657.45 Da
• Isoelectric Point: 10.3584

>T148015 WP_000074043.1 NZ_CP047807:2215345-2215611 [Staphylococcus aureus]
MSNYTVKIKNSAKLDLKKIKHPYLKKTFIEIVETLKNNPYKITQSFEKLEPKYLERYSRRINLQHRVVYTIDDRNKVVLI
LSAWSHYD

Nucleotide

• Length: 267 bp

>T148015 NZ_CP063394:c145184-144903 [Pseudomonas aeruginosa]
ATGAGCCTGAAGTGGACCCGCAAGGCGGCCGCCGACCTGGACACCATCTACGACCATTACGTCGTGCTGATCGGCCCGGA
AAAAGCTCTGAAAGCCGTTCAGGACATCGTCGAGCAGGTGAAACCGCTGCAGCAGGTAGCCAACCAGGGGGCAGGGCGGC
CCAGCGAGGTGCCAGGCGTACGCACCCTGACCCTGGAGCGCTGGCCGTTCAGCGCCCCGTTTCGGGTTAAAGGCAAGGAA
ATCCAGATTTTGCGCATCGACAGGGTCGAAATTACCCCCTGA

Antitoxin

Protein

• Length: 86 a.a.
• Molecular weight: 9762.78 Da
• Isoelectric Point: 4.1958

>AT148015 WP_000587618.1 NZ_CP047807:2215088-2215345 [Staphylococcus aureus]
MIITSPTEARKDFYQLLKNVNNNHEPIYISGNNAENNAVIIGLEDWKSIQETMYLVSTGTMDKVREREKDNSGTTNIDDI
DWDNL

Nucleotide

• Length: 258 bp

>AT148015 NZ_CP063394:c145408-145181 [Pseudomonas aeruginosa]
ATGAGCACCGTAGTCTCGTTCCGCGCCGATGACGCCCTGGTCGCGGCCCTCGACGAACTGGCCCGCGCCACCCACCGCGA
CCGCCCCTACCACCTGCGGCAGGCGCTCGCGCAGTACCTGGAAAGGCAGCAGTGGCAGGTCGCTGCCATCGATGAAGGCT
TGGCCGATGCCAATGCCGGTCGCCTGCTGGAACACATCGAGATCGAGAAGCGCTGGGGGCTGCAATGA

Structures

Toxin

• AlphaFold DB: T1YCD0

Antitoxin

• AlphaFold DB: T1YBB2