Detailed information of TA system TA147827

Bioinformatically predicted

Overview

TA module

• Type: I
• Classification (family/domain): txpA-SprF1/-
• Location: 1963103..1963410
• Replicon: chromosome
• Accession: NZ_CP047795
• Organism: Staphylococcus aureus strain UP_490

Toxin (Protein)

• Gene name: txpA
• Uniprot ID: Q2FWU9
• Locus tag: E3S79_RS09600
• Protein ID: WP_011447039.1
• Coordinates: 1963234..1963410 (-)
• Length: 59 a.a.

Antitoxin (RNA)

Gene name	SprF1
Locus tag	-
Coordinates	1963103..1963242 (+)

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3S79_RS09560	1958442..1958702	+	261	WP_001791826.1	hypothetical protein	-
E3S79_RS09565	1958755..1959105	-	351	WP_000702263.1	complement inhibitor SCIN-A	-
E3S79_RS09570	1959790..1960239	+	450	WP_000727649.1	chemotaxis-inhibiting protein CHIPS	-
E3S79_RS09575	1960334..1960669	-	336	Protein_1841	SH3 domain-containing protein	-
E3S79_RS09580	1961319..1961810	-	492	WP_000919350.1	staphylokinase	-
E3S79_RS09585	1962001..1962756	-	756	WP_000861038.1	CHAP domain-containing protein	-
E3S79_RS09590	1962768..1963022	-	255	WP_000611512.1	phage holin	-
E3S79_RS09595	1963074..1963181	+	108	WP_001791821.1	hypothetical protein	-
-	1963103..1963242	+	140	NuclAT_0	-	Antitoxin
-	1963103..1963242	+	140	NuclAT_0	-	Antitoxin
-	1963103..1963242	+	140	NuclAT_0	-	Antitoxin
-	1963103..1963242	+	140	NuclAT_0	-	Antitoxin
E3S79_RS09600	1963234..1963410	-	177	WP_011447039.1	putative holin-like toxin	Toxin
E3S79_RS09605	1963560..1963856	-	297	WP_000539688.1	DUF2951 domain-containing protein	-
E3S79_RS09610	1963914..1964186	-	273	WP_064138970.1	hypothetical protein	-
E3S79_RS09620	1965360..1965758	-	399	WP_064140498.1	hypothetical protein	-
E3S79_RS09625	1965771..1966175	-	405	WP_000401960.1	RusA family crossover junction endodeoxyribonuclease	-
E3S79_RS09630	1966184..1966402	-	219	WP_000338530.1	hypothetical protein	-
E3S79_RS09635	1966409..1967302	-	894	WP_000148321.1	DnaD domain-containing protein	-
E3S79_RS09640	1967332..1967802	-	471	WP_000610648.1	single-stranded DNA-binding protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	scn / chp / sak / hlb / groEL	1958755..1988439	29684
	-	flank	IS/Tn	-	-	1965038..1965319	281

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Small toxic protein BsrE-like (30-58)

Sequences

Toxin

Protein

• Length: 59 a.a.
• Molecular weight: 6849.45 Da
• Isoelectric Point: 10.6777

>T147827 WP_011447039.1 NZ_CP047795:c1963410-1963234 [Staphylococcus aureus]
MDRWWLSEYKEVVPMVALLKSLERRRLMITISTMLQFGLFLIALIGLVIKLIELSNKK

Nucleotide

• Length: 177 bp

>T147827 NZ_CP063332:c1073869-1073615 [Escherichia coli]
GTGAAACTAATCTGGTCTGAGGAATCATGGGATGATTATCTGTACTGGCAGGAAACAGATAAGCGAATTGTTAAAAAGAT
CAATGAACTTATCAAAGATACCCGCAGAACACCATTTGAAGGTAAGGGGAAACCAGAACCCCTGAAACATAATTTGTCAG
GTTTCTGGTCCCGACGCATTACAGAGGAGCACCGTCTGGTATACGCGGTTACTGATGATTCACTGCTCATTGCAGCATGT
CGTTATCATTATTGA

Antitoxin

Nucleotide

• Length: 140 bp

>AT147827 NZ_CP047795:1963103-1963242 [Staphylococcus aureus]
ATATATAGAAAAAGGGCAACATGCGCAAACATGTTACCCTAATGAGCCCGTTAAAAAGACGGTGGCTATTTTAGATTAAA
GATTAAATTAATAACCATTTAACCATCGAAACCAGCCAAAGTTAGCGATGGTTATTTTTT

Structures

Toxin

• PDB: 7NS1

Antitoxin