Detailed information of TA system TA147638

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2943006..2943420
• Replicon: chromosome
• Accession: NZ_CP047782
• Organism: Staphylococcus aureus strain UP_1352

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A6B5KJM6
• Locus tag: E3T04_RS14740
• Protein ID: WP_001065110.1
• Coordinates: 2943006..2943257 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: E3T04_RS14885
• Protein ID: WP_000028424.1
• Coordinates: 2943247..2943420 (+)
• Length: 58 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T04_RS14685	2938138..2938689	+	552	WP_000704705.1	single-stranded DNA-binding protein	-
E3T04_RS14690	2938702..2939394	+	693	WP_073396995.1	hypothetical protein	-
E3T04_RS14695	2939366..2940169	+	804	WP_160200009.1	phage replisome organizer N-terminal domain-containing protein	-
E3T04_RS14700	2940179..2940958	+	780	WP_000803070.1	ATP-binding protein	-
E3T04_RS14705	2940952..2941110	+	159	WP_000256597.1	hypothetical protein	-
E3T04_RS14710	2941123..2941344	+	222	WP_001123688.1	DUF3269 family protein	-
E3T04_RS14715	2941355..2941759	+	405	WP_000049786.1	DUF1064 domain-containing protein	-
E3T04_RS14720	2941764..2941949	+	186	WP_001187250.1	DUF3113 family protein	-
E3T04_RS14725	2941950..2942255	+	306	WP_000101252.1	hypothetical protein	-
E3T04_RS14730	2942383..2942742	+	360	WP_000029372.1	hypothetical protein	-
E3T04_RS14735	2942743..2942991	+	249	WP_001126839.1	phi PVL orf 51-like protein	-
E3T04_RS14740	2943006..2943257	+	252	WP_001065110.1	DUF1024 family protein	Toxin
E3T04_RS14885	2943247..2943420	+	174	WP_000028424.1	hypothetical protein	Antitoxin
E3T04_RS14745	2943421..2943702	+	282	WP_000454994.1	hypothetical protein	-
E3T04_RS14890	2943703..2943861	+	159	WP_000889681.1	hypothetical protein	-
E3T04_RS14750	2943879..2944412	+	534	WP_001061840.1	dUTPase	-
E3T04_RS14755	2944449..2944622	+	174	WP_001209216.1	hypothetical protein	-
E3T04_RS14760	2944639..2944875	+	237	WP_000195761.1	DUF1381 domain-containing protein	-
E3T04_RS14765	2944900..2945136	+	237	WP_000483477.1	hypothetical protein	-
E3T04_RS14770	2945129..2945278	+	150	WP_000595234.1	transcriptional activator RinB	-
E3T04_RS14775	2945437..2946086	+	650	Protein_2873	hypothetical protein	-
E3T04_RS14780	2946086..2946286	+	201	WP_000265041.1	DUF1514 family protein	-
E3T04_RS14785	2946309..2946779	+	471	WP_000282748.1	hypothetical protein	-
E3T04_RS14790	2946894..2947346	+	453	WP_000406186.1	nucleoside triphosphate pyrophosphohydrolase family protein	-
E3T04_RS14795	2947362..2947718	+	357	WP_102785854.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	2928655..2947718	19063

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9405.32 Da
• Isoelectric Point: 3.8051

>T147638 WP_001065110.1 NZ_CP047782:2943006-2943257 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKARAFDEILEGLPNAMQDALKEDIGLDEAVGIMTGQVVYKYEEEQE
NEY

Nucleotide

• Length: 252 bp

>T147638 NZ_CP063155:73723-74103 [Escherichia coli]
ATGAGGCATATATCACCGGAAGAACTTATTGCGCTTCATGATGCGAATATAAACCGCTACGGCGGCCTGCCTGGCATGTC
AGATCCGGGCAGGGCAGAGGCCATTATCGGGAGAGTCCAGGCCAGAGTTGTGTACGAAGAGATTACCGACCTTTTCGAAG
TCTCCGCAACCTACCTGGTGGCTACTGCGAGAGGGCATATATTCAATGATGCCAATAAGCGTACCGCGCTAAACAGTGCG
CTGTTATTTCTACGCCGTAACGGGGTGCAGGTATTTGATTCACCTGAACTGGCAGACCTTACCGTAGGGGCTGCGACCGG
AGAGATATCTGTATCTTCTGTCGCCGACACGTTACGTAGATTGTATGGTTCCGCGGAGTAG

Antitoxin

Protein

• Length: 58 a.a.
• Molecular weight: 6468.29 Da
• Isoelectric Point: 4.2484

>AT147638 WP_000028424.1 NZ_CP047782:2943247-2943420 [Staphylococcus aureus]
MSISVGDKVYNHETNESLEIVQLVGDIRDTHYKLSDDSVISIIDFITKPIYLIKGDE

Nucleotide

• Length: 174 bp

>AT147638 NZ_CP063155:73502-73723 [Escherichia coli]
ATGCAATCCATTAACTTCCGTACCGCGCGCGGCAACCTTTCTGAAGTGCTCAACAATGTTGAAGCCGGGGAAGAGGTTGA
AATCACCCGCAGAGGCCGTGAGCCAGCAGTAATTGTCAGCAAGGCTACTTTCGAAGCCTACAAAAAAGCGGCGCTGGATG
CTGAATTTGCATCCCTGTTTGACACCCTGGACTCCACCAACAAGGAACTGGTTAACCGATAA

Structures

Toxin

• AlphaFold DB: A0A6B5KJM6