Detailed information of TA system TA147617

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/-
• Location: 2946597..2947020
• Replicon: chromosome
• Accession: NZ_CP047781
• Organism: Staphylococcus aureus strain UP_1452

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A5S9I4Y6
• Locus tag: E3T07_RS14870
• Protein ID: WP_001065091.1
• Coordinates: 2946597..2946848 (+)
• Length: 84 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A0E0VQM8
• Locus tag: E3T07_RS14875
• Protein ID: WP_000028422.1
• Coordinates: 2946838..2947020 (+)
• Length: 61 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T07_RS14830	2942252..2943172	+	921	WP_000138472.1	recombinase RecT	-
E3T07_RS14835	2943253..2943870	+	618	WP_071621397.1	MBL fold metallo-hydrolase	-
E3T07_RS14840	2943871..2944341	+	471	WP_000934764.1	single-stranded DNA-binding protein	-
E3T07_RS14845	2944371..2945255	+	885	WP_000148316.1	DnaD domain protein	-
E3T07_RS14850	2945262..2945480	+	219	WP_000338531.1	hypothetical protein	-
E3T07_RS14855	2945489..2945798	+	310	Protein_2888	RusA family crossover junction endodeoxyribonuclease	-
E3T07_RS14860	2945811..2946179	+	369	WP_000101274.1	hypothetical protein	-
E3T07_RS14865	2946183..2946425	+	243	WP_000131366.1	hypothetical protein	-
E3T07_RS15025	2946437..2946604	+	168	WP_001624242.1	hypothetical protein	-
E3T07_RS14870	2946597..2946848	+	252	WP_001065091.1	DUF1024 family protein	Toxin
E3T07_RS14875	2946838..2947020	+	183	WP_000028422.1	hypothetical protein	Antitoxin
E3T07_RS14880	2947013..2947555	+	543	WP_000185659.1	dUTP pyrophosphatase	-
E3T07_RS14885	2947592..2947798	+	207	WP_000195803.1	DUF1381 domain-containing protein	-
E3T07_RS14890	2947795..2948181	+	387	WP_000592207.1	hypothetical protein	-
E3T07_RS14895	2948178..2948327	+	150	WP_000595265.1	transcriptional activator RinB	-
E3T07_RS14900	2948327..2948527	+	201	WP_000265043.1	DUF1514 family protein	-
E3T07_RS14905	2948555..2948971	+	417	WP_000590122.1	hypothetical protein	-
E3T07_RS14910	2949203..2949502	+	300	WP_000988336.1	HNH endonuclease	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	hld / groEL / tsst-1 / hlb	2889128..2949502	60374

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Protein of unknown function DUF1024 (1-82)

Antitoxin

No domain identified.

Sequences

Toxin

Protein

• Length: 84 a.a.
• Molecular weight: 9349.22 Da
• Isoelectric Point: 3.9473

>T147617 WP_001065091.1 NZ_CP047781:2946597-2946848 [Staphylococcus aureus]
MNNREQIEQSVISASAYNGNDTEGLLKEIEDVYKKAQAFDEILEGMTNAIQHSVKEGVELDEAVGIMAGQVVYKYEEEQE
NEH

Nucleotide

• Length: 252 bp

>T147617 NZ_CP063153:374664-375014 [Escherichia coli]
ATGGTAAAGAAAAGTGAATTTGAACGGGGAGACATTGTGCTGGTTGGCTTTGATCCAGCAAGCGGCCATGAACAGCAAGG
TGCTGGTCGACCTGCGCTTGTGCTCTCCGTTCAAGCCTTTAATCAACTGGGAATGACGCTGGTGGCCCCCATTACGCAGG
GCGGAAATTTTGCCCGTTATGCCGGATTTAGCGTTCCTTTACATTGCGAAGAAGGCGATGTGCACGGCGTGGTGCTGGTG
AATCAGGTGCGGATGATGGATCTACGCGCCCGGCTGGCAAAGCGTATTGGTCTGGCTGCGGGTGAGGTGGTGGAAGAGGC
GTTATTACGTTTGCAGGCGGTGGTGGAATAA

Antitoxin

Protein

• Length: 61 a.a.
• Molecular weight: 6741.63 Da
• Isoelectric Point: 4.2658

>AT147617 WP_000028422.1 NZ_CP047781:2946838-2947020 [Staphylococcus aureus]
MSISVGDKVFNPETNSTLEIVQLVGDIRDTHYKLSDGSIISLIDFVVKPIHLIKEEQEND

Nucleotide

• Length: 183 bp

>AT147617 NZ_CP063153:374419-374670 [Escherichia coli]
ATGCGTATTACCATAAAAAGATGGGGGAACAGTTCAGGTATGGTCATTCCTAATGTTGTGATGAAAGAACTTAACTTACG
GCCGGGGCAGAGCGTGGAGGCGCAAGTGAGCAACAATCAACTGATTCTGACACCCATTTCCAGGCGCTACTCGCTTGATG
AACTGCTGGCACAGTGTGACATGAACGCCACGGAACTTAGCGAGCAGGATGTCTGGGGTAAATCCACCCCTGCGGGTGAC
GAAATATGGTAA

Structures

Toxin

• AlphaFold DB: A0A5S9I4Y6

Antitoxin

• AlphaFold DB: A0A0E0VQM8