Detailed information of TA system TA147532

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): yefM-yoeB (relBE)/Txe-RelB
• Location: 2393869..2394386
• Replicon: chromosome
• Accession: NZ_CP047777
• Organism: Staphylococcus aureus strain UP_1632

Toxin (Protein)

• Gene name: yoeB
• Uniprot ID: A0A3A3ATA4
• Locus tag: E3T16_RS11860
• Protein ID: WP_000113265.1
• Coordinates: 2394120..2394386 (+)
• Length: 89 a.a.

Antitoxin (Protein)

• Gene name: yefM
• Uniprot ID: T1YB60
• Locus tag: E3T16_RS11855
• Protein ID: WP_000584499.1
• Coordinates: 2393869..2394120 (+)
• Length: 84 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
E3T16_RS11825	2389567..2390286	+	720	WP_000479576.1	amino acid ABC transporter permease	-
E3T16_RS11830	2390283..2391014	+	732	WP_000615461.1	amino acid ABC transporter ATP-binding protein	-
E3T16_RS11835	2391111..2392361	-	1251	WP_001012225.1	FemA/FemB family glycyltransferase FmhA	-
E3T16_RS11840	2392616..2392978	+	363	WP_000819843.1	DUF4467 domain-containing protein	-
E3T16_RS11845	2392997..2393596	+	600	WP_000162810.1	DsbA family protein	-
E3T16_RS11850	2393603..2393704	+	102	Protein_2293	hypothetical protein	-
E3T16_RS11855	2393869..2394120	+	252	WP_000584499.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
E3T16_RS11860	2394120..2394386	+	267	WP_000113265.1	Txe/YoeB family addiction module toxin	Toxin
E3T16_RS11865	2394421..2394492	+	72	Protein_2296	hypothetical protein	-
E3T16_RS11870	2394681..2396228	+	1548	WP_000727772.1	zinc ABC transporter substrate-binding lipoprotein AdcA	-
E3T16_RS11875	2396414..2396881	+	468	WP_000153347.1	SRPBCC domain-containing protein	-
E3T16_RS11880	2396965..2397147	+	183	WP_000230292.1	hypothetical protein	-
E3T16_RS11885	2397364..2398188	+	825	WP_000572038.1	formate/nitrite transporter family protein	-
E3T16_RS11890	2398422..2398958	+	537	WP_000169317.1	GNAT family N-acetyltransferase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, RelE/ParE toxin family (6-81)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-74)

Sequences

Toxin

Protein

• Length: 89 a.a.
• Molecular weight: 10436.96 Da
• Isoelectric Point: 10.0573

>T147532 WP_000113265.1 NZ_CP047777:2394120-2394386 [Staphylococcus aureus]
MARLNITFSPQAFEDYKYFQQNNKKMVKKINELLKSIDRNGALEGIGKPEKLKSNLTGYYSRRINHEHRLVYTVDDNHIK
IASCKYHY

Nucleotide

• Length: 267 bp

>T147532 NZ_CP063023:3940053-3940271 [Klebsiella pneumoniae]
ATGTCTGATAAGCCATTAACTAAAACAGACTATTTGATGCGCTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGCGT
CATTGAAAAAAATAAATATGAACTGTCTGATAATGAGCTGGCGGTATTTTACTCAGCTGCCGACCATCGTCTGGCGGAAC
TGACCATGAATAAGCTATACGATAAAATCCCGACCTCTGTCTGGAAATTTATCCGCTAA

Antitoxin

Protein

• Length: 84 a.a.
• Molecular weight: 9433.60 Da
• Isoelectric Point: 4.3407

>AT147532 WP_000584499.1 NZ_CP047777:2393869-2394120 [Staphylococcus aureus]
MIIKNYSYARQNLKALMTKVNDDSDMVTVTSTDDKNVVIMSESDYNSMMETLYLQQNPNNAEHLAQSIADLERGKTITKD
IDV

Nucleotide

• Length: 252 bp

>AT147532 NZ_CP063023:3939652-3940026 [Klebsiella pneumoniae]
ATGGACGAATACTCGCCAAAAAGACATGATATTGCACAGCTACGCTTTCTCTGCGAAACGCTGTATCATGACTGCCTTGC
AAACCTGGAAGAGAGCAACCATGGCTGGGTCAACGACCCGACGTCGGCTGTCAATCTCCAGTTAAATGAATTGATCGAGC
ACATTGCCACATTCGCTCTTAATTATAAAATTAAGTATGCAGAGGATAATAAGCTGATCGCTCAGGTTGACGAATATCTG
GACGACACTTTTACGTTGTTCAGTAACTACGGCATTAACTCTACGGATTTGCAAAAGTGGAAAAAATCCGGTAACCGACT
ATTTCGTTGCTTTGTCAACGCCAGCCGGGAAAACCCGGCCAGTCTATCGTGTTAG

Structures

Toxin

• PDB: 7BWF

Antitoxin

• AlphaFold DB: T1YB60