Detailed information of TA system TA147493

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3620400..3621075
• Replicon: chromosome
• Accession: NZ_CP047757
• Organism: Enterobacter hormaechei strain Eho-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GVI63_RS17495
• Protein ID: WP_032619177.1
• Coordinates: 3620400..3620699 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: GVI63_RS17500
• Protein ID: WP_015571639.1
• Coordinates: 3620710..3621075 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI63_RS17475	3615532..3616149	-	618	WP_032634496.1	DUF1819 family protein	-
GVI63_RS17480	3616313..3617281	-	969	WP_080288382.1	IS5-like element IS903B family transposase	-
GVI63_RS17485	3617314..3618969	+	1656	Protein_3417	ATP-binding cassette domain-containing protein	-
GVI63_RS17490	3618989..3620149	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GVI63_RS17495	3620400..3620699	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVI63_RS17500	3620710..3621075	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
GVI63_RS17505	3621102..3622283	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
GVI63_RS17510	3622304..3623191	-	888	WP_032619173.1	LysR family transcriptional regulator	-
GVI63_RS17515	3623289..3623891	+	603	WP_017382759.1	short chain dehydrogenase	-
GVI63_RS17520	3623888..3624619	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3616313..3642030	25717
	-	flank	IS/Tn	-	-	3616313..3617236	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T147493 WP_032619177.1 NZ_CP047757:3620400-3620699 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T147493 NZ_CP063008:353016-353363 [Klebsiella pneumoniae]
ATGTGGGATGTAGAAACGACGGATACGTTTGATGCCTGGTTCGAATTACAAAGTAGAGCTTTAAAAGAGGATATGTTGGC
CACGATGCTCATCCTGTCTGAGTTTGCTCCGCAGCTTGGGCGCCCGTATGTTGATACGGTGAAAGATTCAACGTTTCAGA
ATATGAAAGAACTGCGGGTTCAGCATCATGGGCTCCCCATCCGCGCCTGTTTTGCTTTTGATCCTCTGCGGAAAGCTATC
GTTTTATGTGTGGGCAATAAGGATGGCATGAATGAGAAACGTTTTTACAAAGAGATGATTACTCTGGCTGACAGAGAATT
CAGCCAACACCTGACTAAGGAGCGATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT147493 WP_015571639.1 NZ_CP047757:3620710-3621075 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT147493 NZ_CP063008:353363-353662 [Klebsiella pneumoniae]
ATGGCCACGCTAAAAGAACTCATGGCGAAACAGAGTCCTGATAGCCAGCAGCGTATTGCAGCGAAAGCGGCAGAGATACG
CCAGTCTGTCGCTTTAAACTTGCTTCGAGAAGAGTTGCAAATGTCGCAAACAGAGATGGCTGCGGCTATGGGGGTAAAGC
AGCCAACGATCGCTAAAATGGAACAGGCTGATAACGACCCTCGGCTTTCGACCCTAAAACGCTACATTGCGGCATTGGGG
GGAGAACTCAGTATAAATGTCACATTGCCGACTGGAAAAAAAGTCTCGTTTAATTTGTGA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9