Detailed information of TA system TA147480

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 36832..37562
• Replicon: chromosome
• Accession: NZ_CP047757
• Organism: Enterobacter hormaechei strain Eho-2

Toxin (Protein)

• Gene name: higB
• Uniprot ID: A0A837FIL2
• Locus tag: GVI63_RS00175
• Protein ID: WP_023302825.1
• Coordinates: 36832..37146 (+)
• Length: 105 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GVI63_RS00180
• Protein ID: WP_023302826.1
• Coordinates: 37143..37562 (+)
• Length: 140 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI63_RS00150	32498..33583	+	1086	Protein_29	cellulase family glycosylhydrolase	-
GVI63_RS00155	33489..34673	-	1185	WP_015570118.1	multidrug efflux MFS transporter EmrD	-
GVI63_RS00160	34849..35682	-	834	WP_032620535.1	DMT family transporter	-
GVI63_RS00165	35745..36191	-	447	WP_003861064.1	GNAT family N-acetyltransferase	-
GVI63_RS00170	36256..36345	-	90	WP_017383683.1	type I toxin-antitoxin system toxin TisB	-
GVI63_RS00175	36832..37146	+	315	WP_023302825.1	type II toxin-antitoxin system HigB family toxin	Toxin
GVI63_RS00180	37143..37562	+	420	WP_023302826.1	helix-turn-helix domain-containing protein	Antitoxin
GVI63_RS00185	37709..37807	+	99	WP_057979964.1	ilvB operon leader peptide IvbL	-
GVI63_RS00190	37914..39602	+	1689	WP_023302827.1	acetolactate synthase large subunit	-
GVI63_RS00195	39606..39893	+	288	WP_015570113.1	acetolactate synthase small subunit	-
GVI63_RS00200	40019..40612	+	594	WP_003861054.1	transcriptional regulator UhpA	-
GVI63_RS00205	40609..42114	+	1506	WP_032634219.1	signal transduction histidine-protein kinase/phosphatase UhpB	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin-antitoxin system, mRNA interferase HigB (21-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (84-136)

Sequences

Toxin

Protein

• Length: 105 a.a.
• Molecular weight: 12415.51 Da
• Isoelectric Point: 10.2825

>T147480 WP_023302825.1 NZ_CP047757:36832-37146 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Nucleotide

• Length: 315 bp

>T147480 NZ_CP047757:36832-37146 [Enterobacter hormaechei]
ATGCATTTAATCTCAATGAAGGCAATTTTGGATGCGGTAAGCCAGTTCCCGCAACACAGAGAAGAGCTTCTCTTTTTAGG
ACGAGTTATTGAAAAGAGCCACTGCCCGACGCCCGCTGCACTGAGAAAACTTTTCCCAACGCTGGATAACTTCAAGTATC
TTGATAAGCATTATGTTATTGATATTGCAAATAACAATCTCAGAGTAGTGGCTCTCATATTCTTTGAAAGCCAAAAGTTT
TATGTGCGCCATGTTTTTACTCATAAGGAATATGACCGTTTTACGGAGAAACATCGCACTAAGGGGAAGAAATGA

Antitoxin

Protein

• Length: 140 a.a.
• Molecular weight: 15380.96 Da
• Isoelectric Point: 5.2068

>AT147480 WP_023302826.1 NZ_CP047757:37143-37562 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Nucleotide

• Length: 420 bp

>AT147480 NZ_CP047757:37143-37562 [Enterobacter hormaechei]
ATGATGATCGTCGCTGATGCAATGAAAGCTACGCACGCCCTTGTTGCGGCCGTTCCTTTACTGGGTGAACAGCCGAGCGA
GAAAGATTATAAGGATGCGCTTGAGCTGGTTGAGTATCTCCTTATGAACGAGCCCAATAGTCCTTTGCTGGATATTGTGT
GCGCCAGAATTAGCCATTACGAAGCTAATGGGCCAGAGATAGTCGCATTACGCAAGGAGATGGAGTCCGTTCCCGTCGGA
ATTGCAGTTTTAAGAACCTTGATGGATCAATACAATCTGACGATATCGGATTTTAAGGATGAAATTGGTAGTAAATCGAT
GGTTTCACGGGTTCTGAATGGTCAGAGACAGCTTACCCTGAACCATATTAAAAAGCTGGCGGCCAGATTTGGGGTATCAC
CTGCGTTATTTATTGAGTGA

Structures

Toxin

• AlphaFold DB: A0A837FIL2