Detailed information of TA system TA147475

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3621865..3622540
• Replicon: chromosome
• Accession: NZ_CP047740
• Organism: Enterobacter hormaechei strain Eho-4

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GVI65_RS17505
• Protein ID: WP_032619177.1
• Coordinates: 3621865..3622164 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: GVI65_RS17510
• Protein ID: WP_015571639.1
• Coordinates: 3622175..3622540 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI65_RS17485	3616997..3617614	-	618	WP_032634496.1	DUF1819 family protein	-
GVI65_RS17490	3617778..3618746	-	969	WP_080288382.1	IS5-like element IS903B family transposase	-
GVI65_RS17495	3618779..3620434	+	1656	Protein_3419	ATP-binding cassette domain-containing protein	-
GVI65_RS17500	3620454..3621614	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GVI65_RS17505	3621865..3622164	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVI65_RS17510	3622175..3622540	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
GVI65_RS17515	3622567..3623748	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
GVI65_RS17520	3623769..3624656	-	888	WP_032619173.1	LysR family transcriptional regulator	-
GVI65_RS17525	3624754..3625356	+	603	WP_017382759.1	short chain dehydrogenase	-
GVI65_RS17530	3625353..3626084	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	flank	IS/Tn	-	-	3617778..3618701	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T147475 WP_032619177.1 NZ_CP047740:3621865-3622164 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T147475 NZ_CP062997:c4862909-4861992 [Klebsiella pneumoniae]
ATGGATATACAGGCGCCTTATTATCGGCAGGTTGCTCTGCTAATGCAGGTTTTACCCTATGTTGCTGTCGAACGGGAATT
TGCGCTGAAAGGTGGCACTGCAATCAATCTCTTTATCCGCGATTTCCCTCGATTATCGGTGGATATCGACCTCGCCTGGG
TGCCCCTGGAAAGCAGAGCCATCGCTTTACCTCATATACGGGATGCGCTTGCACGTATTGCCGCCAATCTGCAACAACAG
GCAGGTATGAGCGCTGTATTACAAGCTAATCGTTCAGATGAGATGCGTGTCATTGTTACTACCGATAGCGCACAAATAAA
GATAGAGGTTTCACCGGTCGCCCGGGGAACATTATATCCACCACAGGAGCGAGAAGTGGTGGGGGAGGTAGAGGACGTTT
TTGGCTATGCCAGCCTTCCAGTGGTTTCGTTGCCTGACCTCTACGGTGGAAAATTATGCGCGGCGCTGGATCGCCAGCAT
CCCAGAGATTTTTATGACGTGAAATTACTTCTTGATGCGCAGGAGCTTGACCGTCCCATTTTCAATGGGTTTATCGCGTA
TTTACTGAGCCATAATCGGCCTCTGGCTGAAGTGCTGAGTCCTCGCTGGAAAGATATTGCTGAACCTTTTTACCGTGAAT
TCAGCGGGATGACATTTGAGACGATAGCCCTGGAGGAACTGACAGCAGTCCCCAACAGGATGATTGCTGCGTTAAAATCC
TGTTTCACACAACAGGATGTCTATTTTTTACTGTCATTTAAACGAGGCGAACCAGACTGGCGTTTAGCGCCGGAAATGCG
TATTCAGGATTTGCCTGCCGTGCAGTGGAAATTGCGCAATATTCATCAGATGCCAGCGATAAAACGCGCTGAGTCATTAG
ATAAACTGGAAAAGGTGCTGGCCGAGTGGCGCAGTTAG

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT147475 WP_015571639.1 NZ_CP047740:3622175-3622540 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT147475 NZ_CP062997:c4863673-4862915 [Klebsiella pneumoniae]
ATGGCAACAAAATTAAACTGGCTGCTGCAAAACAGCGCTCCCGGAGATGTGATCCTTCAGTCCTGGTTAAGCCAGCATGC
CATCTCTCCCTCTCTGGCCTTTAAATACGCGCAAAGTGGCTGGTTAAAAAAAAGAGGCAACGGCGTATATGCGCGTGCGG
GACGTGAGCCGGAGTGGAACGATGCATTAGCATGTTTGCAAAATCAGCTTGCCGCTCCGGTGTATGTTGCGGGGCTCAGT
AGCTTAGTCTGGCAGGGCCGTTCACACTATCTACAGCTAAAACAAAACCAGTGCTGGCTTAGTGTGGAAAATAAAGCGCT
GCTGCCGAAATGGTTCAGGGAATTCCCGGGTGTGGAGTGGATAGTCATATCGGGGAAAAAACTGCCGGTGCTGGATGATA
GATACCGTGTAACGCTTGATATAAAAGGGAAAAAACTCATCGGCAGTGCGCCAGAACTGGCTGCTTACGAGTTGCTGAGC
GCGGTGCCGGGGACCCTCTCATTTAACCACGCTGCAGAGCTTTTCCAGGGGCTGGTTAATCTTAATCCTCGTAAAGTAGA
GTATCTTCTTTCCGTCAGCCAGTCCGTGCAGGCCAAGCGTTTGTATCTCTTCTTCGCCAGTTTTTACGAGCATGGCTGGC
TCAAACGAATCGATAGCCAAAAAATCGATCTGGGAGCGGGCAAGCGGCAAATCGTGGAAAATGGAAAGTTCAATGCTCAA
TACCAAATCACAGTGCCTGAACGCTTTCAAAAGGAATAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9