Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/COG4683-HTH_37
Location 2995248..2995908 Replicon chromosome
Accession NZ_CP047740
Organism Enterobacter hormaechei strain Eho-4

Toxin (Protein)


Gene name higB Uniprot ID A0A2J0Q4X0
Locus tag GVI65_RS14615 Protein ID WP_017383312.1
Coordinates 2995555..2995908 (-) Length 118 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID A0A2J0Q4Z7
Locus tag GVI65_RS14610 Protein ID WP_017383313.1
Coordinates 2995248..2995550 (-) Length 101 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI65_RS14595 2991422..2992747 + 1326 WP_032619371.1 SidA/IucD/PvdA family monooxygenase -
GVI65_RS14600 2992774..2994963 + 2190 WP_023303946.1 TonB-dependent siderophore receptor -
GVI65_RS14610 2995248..2995550 - 303 WP_017383313.1 helix-turn-helix transcriptional regulator Antitoxin
GVI65_RS14615 2995555..2995908 - 354 WP_017383312.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GVI65_RS14620 2996099..2997049 - 951 WP_032619370.1 HTH-type transcriptional regulator Cbl -
GVI65_RS14625 2997146..2998063 - 918 WP_003859531.1 nitrogen assimilation transcriptional regulator NAC -
GVI65_RS14635 2998595..2999527 - 933 WP_023303950.1 L,D-transpeptidase -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-106)

Antitoxin

HigA2-like, helix-turn-helix domain

(18-89)


Sequences

Toxin        


Download         Length: 118 a.a.        Molecular weight: 13537.37 Da        Isoelectric Point: 9.9538

>T147474 WP_017383312.1 NZ_CP047740:c2995908-2995555 [Enterobacter hormaechei]
VWAIKTTDRFDDWFTSLNDSERASVLAALLVLRERGPGLSRPYADTLKGSRHSNMKELRIQSKGDPLRAFFAFDPNRTGI
VLCAGNKVGNERRFYDEMLLVADREYTRWLNTLKERN

Download         Length: 354 bp

>T147474 NZ_CP062997:c4704939-4704655 [Klebsiella pneumoniae]
ATGACCTATGAACTGGAGTTTGATCCACGAGCCTGGCGCGAATGGCAGAAGCCTGGCGAGACGGTCAAAAAACAGTTCAA
AAATAAGCTCCAGCAGATTGTGCAGAATCTGCGAATTGAGTCGGCCAGGCTGAGCGATTTACCGGATTGCTACAAAATCA
AGCTTAAGGCGTCAGGTTATCGGTTGGTGTATCAAGTACGAGATAGTGTGGTGGTGGTTTACGTTATTGCCATTGGCAAA
AGAGAGAAAGCGGCCGTTTATCATCAGGCGAATAAACGGCTCTAA

Antitoxin


Download         Length: 101 a.a.        Molecular weight: 11028.85 Da        Isoelectric Point: 6.4727

>AT147474 WP_017383313.1 NZ_CP047740:c2995550-2995248 [Enterobacter hormaechei]
MGRTLEQLIADEKPEVVASAQAMATDILLNIHLAELREKVQKTQVDMARALGIKQPTVAVMEKAGRDIKLSSLKRYVEAA
GGKLRLDVELPDGSHYEFVL

Download         Length: 303 bp

>AT147474 NZ_CP062997:c4705171-4704929 [Klebsiella pneumoniae]
ATGGCCACGCTCAATGTTCGACTGGACGATAAACTCAAAAAAGAGGCTTATGCCGTGCTGGAGAAACTCGATATGACACC
AACGGAGGCGGTACGCCTGCTGTTTCAATACATTGCGGAAAATGGCCGCATGCCGGTGAAAAGTATGACCATCAGTGATG
GCGAAGCGGCGTTATTACGCACCGTTCGCGAACGGCTGGAAAACCCACGGCCAGGGGTTAAGGTGAGTCTGGATGACCTA
TGA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A2J0Q4X0

Antitoxin

Source ID Structure
AlphaFold DB A0A2J0Q4Z7

References