Detailed information of TA system TA147457

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-HigA
• Location: 3608526..3609201
• Replicon: chromosome
• Accession: NZ_CP047736
• Organism: Enterobacter hormaechei strain Eho-5

Toxin (Protein)

• Gene name: higB
• Uniprot ID: -
• Locus tag: GVI66_RS17435
• Protein ID: WP_032619177.1
• Coordinates: 3608526..3608825 (+)
• Length: 100 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: A0A2J0PXC9
• Locus tag: GVI66_RS17440
• Protein ID: WP_015571639.1
• Coordinates: 3608836..3609201 (+)
• Length: 122 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI66_RS17415	3603658..3604275	-	618	WP_032634496.1	DUF1819 family protein	-
GVI66_RS17420	3604439..3605407	-	969	WP_000654804.1	IS5-like element IS903B family transposase	-
GVI66_RS17425	3605440..3607095	+	1656	Protein_3406	ATP-binding cassette domain-containing protein	-
GVI66_RS17430	3607115..3608275	+	1161	WP_085929677.1	HlyD family efflux transporter periplasmic adaptor subunit	-
GVI66_RS17435	3608526..3608825	+	300	WP_032619177.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVI66_RS17440	3608836..3609201	+	366	WP_015571639.1	HigA family addiction module antidote protein	Antitoxin
GVI66_RS17445	3609228..3610409	-	1182	WP_032619175.1	PLP-dependent aminotransferase family protein	-
GVI66_RS17450	3610430..3611317	-	888	WP_032619173.1	LysR family transcriptional regulator	-
GVI66_RS17455	3611415..3612017	+	603	WP_017382759.1	short chain dehydrogenase	-
GVI66_RS17460	3612014..3612745	-	732	WP_017382760.1	methyltransferase domain-containing protein	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	-	inside	Prophage	-	-	3604439..3632218	27779
	-	flank	IS/Tn	-	-	3604439..3605362	923

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-93)

Antitoxin

• Cro/C1-type helix-turn-helix domain (25-72)

Sequences

Toxin

Protein

• Length: 100 a.a.
• Molecular weight: 11564.19 Da
• Isoelectric Point: 9.6776

>T147457 WP_032619177.1 NZ_CP047736:3608526-3608825 [Enterobacter hormaechei]
MINHSRDQWLEDFFLYGRSSNVIPLNLETALARKLDIINAAMSHLDLRSPPGNMYEALSPPLKGYSSIRVNRQYRLVFRW
SEGKADDLYLSPHKYTQHK

Nucleotide

• Length: 300 bp

>T147457 NZ_CP062986:c4655716-4655183 [Klebsiella pneumoniae]
ATGGAGCAGCAACTGACGATTGAGATGATTGCCGATGCGTTCAGCTACGATATCACTGGATTTGATTGCGGCGAGGAAGC
GCTGAATACGTTTTTAAAAGAGCACCTCAAGCGGCAGCACGATGGACAAATCTTAAGGGGTTATGCGCTGGTCTCCGGGG
ATACCGTTCCCAGGTTGCTGGGTTATTACACCTTATCGGGGAGTTGTTTCGAGCGAGGCATGTTGCCGTCTAAAACTCAG
CAAAAGAAAATCCCGTATCAAAATGCGCCAAGCGTAACCCTCGGGCGGCTGGCCATTGATAAATCTGTTCAGGGGCAGGG
CTGGGGAGAGATGTTGGTTGCCCATGCGATGCGAGTGGTGTGGGGAGCGTCTAAAGCCGTTGGCATCTACGGATTATTTG
TTGAAGCCCTTAATGAGAAGGCTAAAGCATTCTATCTTCGTCTGGGGTTTATACAGCTCGTGGATGAAAATAGTAACTTA
CTGTTTTATCCGACCAAATCGATTGAACAGCTCTTTACTGACGATGAGTCATAA

Antitoxin

Protein

• Length: 122 a.a.
• Molecular weight: 13705.73 Da
• Isoelectric Point: 9.5936

>AT147457 WP_015571639.1 NZ_CP047736:3608836-3609201 [Enterobacter hormaechei]
MTLQQALRKPTTPGEVLQYEYLEPLNLKINDLAEMLNVHRNTVSALVNNNRKLTADMAIKLAKAFNTTIEFWLNLQLNVD
IWEAQSNSRTQEELSRIKTVAEVMAKRKSGKPDVTLHGNSA

Nucleotide

• Length: 366 bp

>AT147457 NZ_CP062986:c4655993-4655727 [Klebsiella pneumoniae]
ATGCCCGCACTTAAAAAGCAGCGTATAGATTTGAGATTAACCGATGATGATAAGAGCATCATCGAGGAAGCTGCGGCAAT
TTCGAATCAGACGATTACCCAGTTTGTGGTTGCCAGTGCCTCAGAACGTGCTGCGGAAGTGATTGAGCAGCACCGCAGAA
TGGTCCTCAATGAGCAATCATGGAGCCTGGTGATGGAGGCGATTACCCAACCGCCAGCCCCGAATGATCGACTGAAACGT
GCAGCGAAGCGTCTACAAACCAGGTAA

Structures

Antitoxin

• AlphaFold DB: A0A2J0PXC9