Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HigA
Location 36832..37562 Replicon chromosome
Accession NZ_CP047736
Organism Enterobacter hormaechei strain Eho-5

Toxin (Protein)


Gene name higB Uniprot ID A0A837FIL2
Locus tag GVI66_RS00175 Protein ID WP_023302825.1
Coordinates 36832..37146 (+) Length 105 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID -
Locus tag GVI66_RS00180 Protein ID WP_023302826.1
Coordinates 37143..37562 (+) Length 140 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI66_RS00150 32498..33583 + 1086 Protein_29 cellulase family glycosylhydrolase -
GVI66_RS00155 33489..34673 - 1185 WP_015570118.1 multidrug efflux MFS transporter EmrD -
GVI66_RS00160 34849..35682 - 834 WP_032620535.1 DMT family transporter -
GVI66_RS00165 35745..36191 - 447 WP_003861064.1 GNAT family N-acetyltransferase -
GVI66_RS00170 36256..36345 - 90 WP_017383683.1 type I toxin-antitoxin system toxin TisB -
GVI66_RS00175 36832..37146 + 315 WP_023302825.1 type II toxin-antitoxin system HigB family toxin Toxin
GVI66_RS00180 37143..37562 + 420 WP_023302826.1 helix-turn-helix domain-containing protein Antitoxin
GVI66_RS00185 37709..37807 + 99 WP_057979964.1 ilvB operon leader peptide IvbL -
GVI66_RS00190 37914..39602 + 1689 WP_023302827.1 acetolactate synthase large subunit -
GVI66_RS00195 39606..39893 + 288 WP_015570113.1 acetolactate synthase small subunit -
GVI66_RS00200 40019..40612 + 594 WP_003861054.1 transcriptional regulator UhpA -
GVI66_RS00205 40609..42114 + 1506 WP_032634219.1 signal transduction histidine-protein kinase/phosphatase UhpB -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin-antitoxin system, mRNA interferase HigB

(21-93)

Antitoxin

Cro/C1-type helix-turn-helix domain

(84-136)


Sequences

Toxin        


Download         Length: 105 a.a.        Molecular weight: 12415.51 Da        Isoelectric Point: 10.2825

>T147444 WP_023302825.1 NZ_CP047736:36832-37146 [Enterobacter hormaechei]
MHLISMKAILDAVSQFPQHREELLFLGRVIEKSHCPTPAALRKLFPTLDNFKYLDKHYVIDIANNNLRVVALIFFESQKF
YVRHVFTHKEYDRFTEKHRTKGKK

Download         Length: 315 bp

>T147444 NZ_CP062985:c3966705-3966598 [Escherichia coli UTI89]
ATGACGCTCGCAGAACTGGGCATGGCCTTCTGGCATGATTTAGCGGCTCCGGTCATTGCTGGCATTCTTGCCAGTATGAT
CGTGAACTGGCTGAATAAGCGGAAGTAA

Antitoxin


Download         Length: 140 a.a.        Molecular weight: 15380.96 Da        Isoelectric Point: 5.2068

>AT147444 WP_023302826.1 NZ_CP047736:37143-37562 [Enterobacter hormaechei]
MMIVADAMKATHALVAAVPLLGEQPSEKDYKDALELVEYLLMNEPNSPLLDIVCARISHYEANGPEIVALRKEMESVPVG
IAVLRTLMDQYNLTISDFKDEIGSKSMVSRVLNGQRQLTLNHIKKLAARFGVSPALFIE

Download         Length: 420 bp

>AT147444 NZ_CP062985:3966762-3966819 [Escherichia coli UTI89]
TCAAGATTAGCCCCCGTGATGTTGTCAGGTGCATACCTGCAACGTGCGGGGGTTTTCT

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A837FIL2

Antitoxin

Source ID Structure

References