Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/HigB-HTH_XRE
Location 3400025..3400704 Replicon chromosome
Accession NZ_CP047710
Organism Escherichia coli strain Eco-15

Toxin (Protein)


Gene name higB Uniprot ID S1PK60
Locus tag GVI77_RS16280 Protein ID WP_000057523.1
Coordinates 3400402..3400704 (-) Length 101 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID S1QAY3
Locus tag GVI77_RS16275 Protein ID WP_000806442.1
Coordinates 3400025..3400366 (-) Length 114 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI77_RS16265 3396269..3397201 - 933 WP_000883041.1 glutaminase A -
GVI77_RS16270 3397463..3399967 + 2505 WP_000083947.1 copper-exporting P-type ATPase CopA -
GVI77_RS16275 3400025..3400366 - 342 WP_000806442.1 HigA family addiction module antidote protein Antitoxin
GVI77_RS16280 3400402..3400704 - 303 WP_000057523.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GVI77_RS16285 3400837..3401631 + 795 WP_000365147.1 TraB/GumN family protein -
GVI77_RS16290 3401835..3402314 + 480 WP_000186633.1 Cys-tRNA(Pro)/Cys-tRNA(Cys) deacylase YbaK -
GVI77_RS16295 3402338..3403138 + 801 WP_000439798.1 hypothetical protein -
GVI77_RS16300 3403135..3403638 + 504 WP_000667000.1 hypothetical protein -
GVI77_RS16305 3403676..3405328 - 1653 WP_197389265.1 bifunctional UDP-sugar hydrolase/5'-nucleotidase UshA -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-1

(9-98)

Antitoxin

Toxin-antitoxin system, antidote protein, HigA

(1-100)


Sequences

Toxin        


Download         Length: 101 a.a.        Molecular weight: 11809.44 Da        Isoelectric Point: 10.3980

>T147401 WP_000057523.1 NZ_CP047710:c3400704-3400402 [Escherichia coli]
MAQKKNIRSFRDAWLADFFVHSTPHRKIPAEIHTTLSRKLDIINAATSHRDLRSPPGNRYEELSGKLQEYSSIRVNKQYR
LIFKWVNGKAEELFLDPHNY

Download         Length: 303 bp

>T147401 NZ_CP062946:2571238-2571570 [Thalassotalea sp. LPB0316]
ATGGAAGCCATATTTATAGAATCAAGTATTTTTGAAAAACATAGACAAAGTTATATGTCTGATGATGAGTATCGTGCTTT
TCAGCAGGAATTGCTTATCAACCCGTTACAGGGAGATGTTATTCAAGGTACTGGTGGGTTAAGGAAAATTCGAGTAGCCG
CAAAAGGTAAAGGCAAACGAGGAGGGGCAAGAATTATTTATTATTACTTCGTTCAATACCGCCGATTTTATTTACTTACC
GTATACGCCAAAAATGAAATGGCGGATTTAACAGCAAGCCAAAAGGCGCAGTTAAAAGACTTTATGGAGGTCTGGAAAAA
TGAGCAATCGTGA

Antitoxin


Download         Length: 114 a.a.        Molecular weight: 13171.10 Da        Isoelectric Point: 5.7790

>AT147401 WP_000806442.1 NZ_CP047710:c3400366-3400025 [Escherichia coli]
MKQATRKPTTPGDILLYEYLEPLDLKINELAELLHVHRNSVSALINNNRKLTTEMAFRLAKVFDTTVDFWLNLQAAVDLW
EVENNMRTQEELGRIETVAEYLARREERAKKVA

Download         Length: 342 bp

>AT147401 NZ_CP062946:2571557-2571871 [Thalassotalea sp. LPB0316]
ATGAGCAATCGTGATTTATTTTCTGAGTTAACAACTGCCTTGAATGAAGCAAAAGCTCATAGTGAAGGCAAGTTAACGTT
AAAAACACATGAGGTAAAAGAAGCGAGTAATTTGGAGATTAGCCCCCAAGAGATAGTGTCGATAAGAGAGCAATACAATA
TGTCTAGAGGGGTCTTCGCTCGATATCTTCATACGTCTGCACGCACCTTAGAAAACTGGGAACAAGGTCGAAGTGCCCCC
AATGGTCAAGCAGTCACGTTACTCAAACTTGTGCAGACTCACCCTGAAACCCTTTCTCATATTGCATCGCTTTAG

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB S1PK60

Antitoxin

Source ID Structure
PDB 2EBY
AlphaFold DB S1QAY3

References