Detailed information of TA system TA147366

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): phd-doc/Doc-RelB
• Location: 330069..330655
• Replicon: chromosome
• Accession: NZ_CP047707
• Organism: Klebsiella pneumoniae strain Kpn-13

Toxin (Protein)

• Gene name: doc
• Uniprot ID: -
• Locus tag: GVI78_RS01535
• Protein ID: WP_085163455.1
• Coordinates: 330287..330655 (+)
• Length: 123 a.a.

Antitoxin (Protein)

• Gene name: phd
• Uniprot ID: W9B1V1
• Locus tag: GVI78_RS01530
• Protein ID: WP_004174006.1
• Coordinates: 330069..330290 (+)
• Length: 74 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI78_RS01510	326226..327152	+	927	WP_002920807.1	high-affinity branched-chain amino acid ABC transporter permease LivH	-
GVI78_RS01515	327149..328426	+	1278	WP_004174005.1	branched chain amino acid ABC transporter permease LivM	-
GVI78_RS01520	328423..329190	+	768	WP_002920803.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivG	-
GVI78_RS01525	329192..329905	+	714	WP_004145133.1	high-affinity branched-chain amino acid ABC transporter ATP-binding protein LivF	-
GVI78_RS01530	330069..330290	+	222	WP_004174006.1	type II toxin-antitoxin system Phd/YefM family antitoxin	Antitoxin
GVI78_RS01535	330287..330655	+	369	WP_085163455.1	type II toxin-antitoxin system death-on-curing family toxin	Toxin
GVI78_RS01540	330928..332244	+	1317	WP_002920796.1	sn-glycerol-3-phosphate ABC transporter substrate-binding protein UgpB	-
GVI78_RS01545	332351..333238	+	888	WP_002920792.1	sn-glycerol-3-phosphate ABC transporter permease UgpA	-
GVI78_RS01550	333235..334080	+	846	WP_004185988.1	sn-glycerol-3-phosphate ABC transporter permease UgpE	-
GVI78_RS01555	334082..335152	+	1071	WP_004150074.1	sn-glycerol-3-phosphate import ATP-binding protein UgpC	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)
	Similar MGE(s) Similar MGE(s) is defined as other TA-related MGE(s) with Mash distance < 0.01 to this MGE. Detail Genome accession Organism MGE type Related TA Coordinates Mash distance View NC_016845 Klebsiella pneumoniae subsp. pneumoniae HS11286 Prophage TA026031 4967722..4988310 0.006	inside	Prophage	-	-	315175..335889	20714

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Death on curing protein (1-121)

Antitoxin

• Type II toxin-antitoxin system, antitoxin Phd/YefM (1-50)

Sequences

Toxin

Protein

• Length: 123 a.a.
• Molecular weight: 13552.92 Da
• Isoelectric Point: 8.6410

>T147366 WP_085163455.1 NZ_CP047707:330287-330655 [Klebsiella pneumoniae]
MTLQIISAEEIIQFHDRLLRVTPGVAGMPDPGRAEAIMYRVLNKIEYEGVTDVWRLAAMHLLAISRGHIFNDGNKRTALF
ITLLFIKRNGIILPANPDFVGMTVEAAAGQLTLEQIVARLRG

Nucleotide

• Length: 369 bp

>T147366 NZ_CP062916:837680-838090 [Raoultella terrigena]
GTGGTCCTGTGGCAATCTGATTTACGCATTTCCTGGCGTTCGCAGTGGTTCTCGCTGTTGCTCCACGGCATCGTGGCGGC
CATCGTCCTGCTGATGCCCTGGCCGCTCAGCTACACCCCGCTATGGCTACTGCTGCTCTCTCTGGTGGTGTTTGACAGCG
TACGCAGCCAGCGGCGGATCCACGCCTGTCAGGGAGAAATAAAGCTGATGACCGATTCCCGACTGCGCTGGCAAAAAGCG
GAGTGGGAAATAGTCGGCATGCCGTGGGTCATTAACAGCGGGATGCTGCTGCGTCTGCGCCATCCGCAAACGCGTCGCAG
CCAGCACCTGTGGGTGGCGGCTGATAGCATGGATGCGAAGGAGTGGCGCGATTTGCGCCGGCTGGTGCTGAACAAACCGG
CGCAGGACTAG

Antitoxin

Protein

• Length: 74 a.a.
• Molecular weight: 8151.15 Da
• Isoelectric Point: 4.7769

>AT147366 WP_004174006.1 NZ_CP047707:330069-330290 [Klebsiella pneumoniae]
MRTVNYSEARQNLADVLESAVTGVPVTITRRGHKSAVIISAEEFERYQAARMDDEFAAIMAVHGDEIRELADK

Nucleotide

• Length: 222 bp

>AT147366 NZ_CP062916:837433-837699 [Raoultella terrigena]
ATGGACATTACTAATAAAGCCCGCATCCACTGGGCATGCCGCCGGGGTATGCGTGAACTCGATATCTCCATCATGCCGTT
TTTCGAGTATGAGTACGATACGCTCAGCGATGAAGACAAGCTGCTGTTCATCCGGTTGTTAGAAAACGACGACCCGGATC
TGTTCAACTGGTTGATGAATCACGGCGAACCCTCCGATGCCCAGCTGCAGCGGATGGTAAAATTAATCCAGACTCGGAAT
CGGGAACGTGGTCCTGTGGCAATCTGA

Structures

Antitoxin

• AlphaFold DB: A0A5E5YJY7