Detailed information of TA system    

insolicoBioinformatically predicted

Overview

TA module


Type II Classification (family/domain) higBA (relBE)/Tad-HTH_37
Location 3892529..3893126 Replicon chromosome
Accession NZ_CP047700
Organism Klebsiella pneumoniae strain Kpn-14

Toxin (Protein)


Gene name higB Uniprot ID R4YIC5
Locus tag GVI79_RS19345 Protein ID WP_004142563.1
Coordinates 3892809..3893126 (-) Length 106 a.a.

Antitoxin (Protein)


Gene name higA Uniprot ID R4YH91
Locus tag GVI79_RS19340 Protein ID WP_004142561.1
Coordinates 3892529..3892816 (-) Length 96 a.a.

Genomic Context

Locus tag Coordinates Strand Size (bp) Protein ID Product Description
GVI79_RS19310 3888623..3888871 + 249 WP_002893037.1 DUF1158 domain-containing protein -
GVI79_RS19315 3888889..3889230 - 342 WP_002893035.1 RamA family antibiotic efflux transcriptional regulator -
GVI79_RS19320 3889261..3890166 - 906 WP_062920869.1 MBL fold metallo-hydrolase -
GVI79_RS19325 3890554..3891123 + 570 WP_062920870.1 TetR/AcrR family transcriptional regulator -
GVI79_RS19330 3891123..3891491 + 369 WP_004142557.1 MmcQ/YjbR family DNA-binding protein -
GVI79_RS19335 3891611..3892264 + 654 Protein_3795 oxygen-insensitive NAD(P)H nitroreductase -
GVI79_RS19340 3892529..3892816 - 288 WP_004142561.1 helix-turn-helix transcriptional regulator Antitoxin
GVI79_RS19345 3892809..3893126 - 318 WP_004142563.1 type II toxin-antitoxin system RelE/ParE family toxin Toxin
GVI79_RS19350 3893311..3894354 - 1044 WP_032419264.1 DUF2157 domain-containing protein -
GVI79_RS19355 3895021..3895887 - 867 WP_032419265.1 helix-turn-helix transcriptional regulator -
GVI79_RS19360 3895996..3897423 + 1428 WP_004176980.1 MFS transporter -

Associated MGEs

MGE
detail		 Similar
MGEs		 Relative
position		 MGE Type Cargo ARG Virulence gene Coordinates Length (bp)


Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.


Domains

Predicted by InterproScan

Toxin

Toxin HigB-like

(12-100)

Antitoxin

Cro/C1-type helix-turn-helix domain

(38-88)


Sequences

Toxin        


Download         Length: 106 a.a.        Molecular weight: 12110.39 Da        Isoelectric Point: 11.2767

>T147358 WP_004142563.1 NZ_CP047700:c3893126-3892809 [Klebsiella pneumoniae]
MFRLVVHVDVKKELQALPPIVQAKMIRQLDKLRQNPTALREPDSKPLGQGLFEIRALGSVQGRGIYVFQQGKTLFLLRVF
VKKTQKTPSSEIRLALKRLEEMRNE

Download         Length: 318 bp

>T147358 NZ_CP062910:4251642-4251977 [Escherichia coli O112ab:H8]
ATGGTAAGCCGATACGTACCCGATATGGGCGATCTGATTTGGGTTGATTTTGACCCGACAAAAGGTAGCGAGCAAGCTGG
ACATCGTCCAGCTGTTGTCCTGAGTCCTTTCATGTACAACAACAAAACAGGTATGTGTCTGTGTGTTCCTTATACAACGC
AATCAAAAGGATATCCGTTCGAAGTTGTTTTATCCGGTCAGGAACGTGATGGCGTAGCGTTAGCTGATCAGGTAAAAAGT
ATCGCCTGGCGGGCAAGAGGAGCAACGAAGAAAGGAACAGTTGCCCCAGAGGAATTACAACTCATTAAAGCCAAAATTAA
CGTACTGATTGGGTAG

Antitoxin


Download         Length: 96 a.a.        Molecular weight: 10771.46 Da        Isoelectric Point: 10.2212

>AT147358 WP_004142561.1 NZ_CP047700:c3892816-3892529 [Klebsiella pneumoniae]
MSKKIIDWDTLKTELLGQPEVQAAFDAQERQQRLRTMLAQWREQAGLTRAQVAQRMGVSAPTVSRMEANITRASLDTLTR
YALACGIKHPQVLLY

Download         Length: 288 bp

>AT147358 NZ_CP062910:4251394-4251642 [Escherichia coli O112ab:H8]
ATGATCCACAGTAGCGTAAAGCGTTGGGGAAATTCACCGGCGGTGCGGATCCCGGCTACGTTAATGCAGGCGCTCAATCT
GAATATTGATGATGAAGTGAAGATTGACCTGGTGGATGGCAAATTAATTATTGAGCCAGTGCGTAAAGAGCCCGTATTTA
CGCTTGCTGAACTGGTCAACGACATCACGCCGGAAAACCTCCACGAGAATATCGACTGGGGAGAGCCGAAAGATAAGGAA
GTCTGGTAA

Similar Proteins

Only experimentally validated proteins are listed.

Protein Organism Identities (%) Coverage (%) Ha-value
Protein Organism Identities (%) Coverage (%) Ha-value

Structures

Toxin

Source ID Structure
AlphaFold DB A0A0M5MXH8

Antitoxin

Source ID Structure
AlphaFold DB A0A0S3DIQ1

References