Detailed information of TA system TA147337

Bioinformatically predicted

Overview

TA module

• Type: II
• Classification (family/domain): higBA (relBE)/HigB-VapI
• Location: 1525664..1526259
• Replicon: chromosome
• Accession: NZ_CP047697
• Organism: Pseudomonas aeruginosa strain RD1-3

Toxin (Protein)

• Gene name: higB
• Uniprot ID: V6ALY3
• Locus tag: GVI48_RS07040
• Protein ID: WP_003113526.1
• Coordinates: 1525664..1525942 (+)
• Length: 93 a.a.

Antitoxin (Protein)

• Gene name: higA
• Uniprot ID: -
• Locus tag: GVI48_RS07045
• Protein ID: WP_003099268.1
• Coordinates: 1525954..1526259 (+)
• Length: 102 a.a.

Genomic Context

Locus tag	Coordinates	Strand	Size (bp)	Protein ID	Product	Description
GVI48_RS07025	1521090..1522328	+	1239	WP_003113524.1	dipeptidase	-
GVI48_RS07030	1522390..1523037	+	648	WP_003095021.1	carbonate dehydratase	-
GVI48_RS07035	1523107..1525335	-	2229	WP_023090880.1	TonB-dependent receptor	-
GVI48_RS07040	1525664..1525942	+	279	WP_003113526.1	type II toxin-antitoxin system RelE/ParE family toxin	Toxin
GVI48_RS07045	1525954..1526259	+	306	WP_003099268.1	HigA family addiction module antidote protein	Antitoxin
GVI48_RS07055	1526655..1527755	-	1101	WP_003099270.1	redox-regulated ATPase YchF	-
GVI48_RS07060	1527796..1528380	-	585	WP_003099278.1	aminoacyl-tRNA hydrolase	-
GVI48_RS07065	1528422..1529036	-	615	WP_003099279.1	50S ribosomal protein L25/general stress protein Ctc	-
GVI48_RS07070	1529153..1530094	-	942	WP_003099281.1	ribose-phosphate pyrophosphokinase	-
GVI48_RS07080	1530261..1531109	-	849	WP_003099284.1	4-(cytidine 5'-diphospho)-2-C-methyl-D-erythritol kinase	-

Associated MGEs

MGE detail	Similar MGEs	Relative position	MGE Type	Cargo ARG	Virulence gene	Coordinates	Length (bp)

Relative position:
(1) inside: TA loci is completely located inside the MGE;
(2) overlap: TA loci is partially overlapped with the MGE;
(3) flank: The TA loci is located in the 5 kb flanking regions of MGE.

Domains

Predicted by InterproScan

Toxin

• Toxin HigB-1 (5-92)

Antitoxin

• Toxin-antitoxin system, antidote protein, HigA (1-97)

Sequences

Toxin

Protein

• Length: 93 a.a.
• Molecular weight: 10646.19 Da
• Isoelectric Point: 7.8937

>T147337 WP_003113526.1 NZ_CP047697:1525664-1525942 [Pseudomonas aeruginosa]
MILTFRCDETRQLFETGLSRRWGAILTVATRKLAMLHAATELRDLRSPPGNRLEPLQGKRAGQHSIRINDQWRVCFVWTD
AGPEEVEIVDYH

Nucleotide

• Length: 279 bp

>T147337 NZ_CP062908:3440881-3441099 [Escherichia coli]
ATGTCCGAAAAACCTTTAACGAAAACCGATTATTTAATGCGTTTACGTCGTTGCCAGACAATTGACACGCTGGAGCGTGT
TATCGAGAAAAATAAATACGAATTATCAGATAATGAACTGGCGGTATTTTACTCAGCCGCAGATCACCGCCTCGCCGAAT
TGACCATGAATAAACTGTACGACAAGATCCCTTCCTCAGTATGGAAATTTATTCGCTAA

Antitoxin

Protein

• Length: 102 a.a.
• Molecular weight: 11213.79 Da
• Isoelectric Point: 5.0747

>AT147337 WP_003099268.1 NZ_CP047697:1525954-1526259 [Pseudomonas aeruginosa]
MATNGMRPIHPGEILRDEFLMELDISPAALARALKVSAPTVNDIVREQRGISADMAIRLGRYFDTSAQFWMNLQSEYSLA
TAYAANGKQIEHEIEPLLAHG

Nucleotide

• Length: 306 bp

>AT147337 NZ_CP062908:3440481-3440855 [Escherichia coli]
ATGGATGAATACTCACCCAAAAGACATGATATCGCACAGCTTAAGTTTCTCTGTGAAACCCTGTATCATGACTGCCTTGC
AAACCTTGAAGAAAGCAATCATGGCTGGGTAAACGACCCAACCTCGGCGATCAACCTCCAGTTGAATGAACTGATTGAGC
ATATTGCGACCTTCGCACTTAATTACAAAATTAAGTATAATGAAGACAATAAGCTCATTGAGCAGATCGACGAATATCTG
GATGACACCTTTATGTTGTTCAGTAGTTATGGTATTAATATGCAGGATCTTCAGAAATGGCGGAAGTCAGGTAATCGACT
ATTCCGTTGTTTTGTCAATGCGACGAAAGAGAATCCTGCGAGTTTATCTTGTTAG

Structures

Toxin

• AlphaFold DB: V6ALY3